Detailed information NTG0475903

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: H8S71_RS12570
• Genome accession: NZ_CP060417
• Coordinates: 2462990..2463607 (-)
• Length: 205 a.a.
• NCBI ID: WP_163182169.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain ONU 559
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2457990..2468607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H8S71_RS12540 (H8S71_12535)	rpsT	2458292..2458558 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
H8S71_RS12545 (H8S71_12540)	holA	2458573..2459616 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
H8S71_RS12550 (H8S71_12545)	-	2459656..2459769 (-)	114	WP_134975484.1	hypothetical protein	-
H8S71_RS12555 (H8S71_12550)	yqzM	2459846..2459980 (+)	135	WP_003229983.1	YqzM family protein	-
H8S71_RS12560 (H8S71_12555)	comEC	2460020..2462350 (-)	2331	WP_187001806.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
H8S71_RS12565 (H8S71_12560)	comEB	2462354..2462923 (-)	570	WP_003229978.1	ComE operon protein 2	-
H8S71_RS12570 (H8S71_12565)	comEA	2462990..2463607 (-)	618	WP_163182169.1	competence protein ComEA	Machinery gene
H8S71_RS12575 (H8S71_12570)	comER	2463691..2464512 (+)	822	WP_032726195.1	late competence protein ComER	-
H8S71_RS12580 (H8S71_12575)	yqeM	2464578..2465321 (-)	744	WP_070547553.1	class I SAM-dependent methyltransferase	-
H8S71_RS12585 (H8S71_12580)	rsfS	2465318..2465674 (-)	357	WP_014480315.1	ribosome silencing factor	-
H8S71_RS12590 (H8S71_12585)	yqeK	2465692..2466252 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H8S71_RS12595 (H8S71_12590)	nadD	2466242..2466811 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
H8S71_RS12600 (H8S71_12595)	yhbY	2466823..2467113 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
H8S71_RS12605 (H8S71_12600)	aroE	2467107..2467949 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21709.44 Da
• Isoelectric Point: 4.8354

>NTDB_id=475903 H8S71_RS12570 WP_163182169.1 2462990..2463607(-) (comEA) [Bacillus subtilis subsp. subtilis strain ONU 559]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGAKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFDKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=475903 H8S71_RS12570 WP_163182169.1 2462990..2463607(-) (comEA) [Bacillus subtilis subsp. subtilis strain ONU 559]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGGCCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGATAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	96.585	100	0.966
comEA	Staphylococcus aureus MW2	36.364	100	0.39
comEA	Staphylococcus aureus N315	35.909	100	0.385