Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   H8S71_RS12560 Genome accession   NZ_CP060417
Coordinates   2460020..2462350 (-) Length   776 a.a.
NCBI ID   WP_187001806.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain ONU 559     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2455020..2467350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8S71_RS12525 (H8S71_12520) yqxA 2455359..2455697 (-) 339 WP_124048123.1 YqxA family protein -
  H8S71_RS12530 (H8S71_12525) spoIIP 2455714..2456919 (-) 1206 WP_014477383.1 spore autolysin SpoIIP -
  H8S71_RS12535 (H8S71_12530) gpr 2456982..2458088 (-) 1107 WP_014480309.1 GPR endopeptidase -
  H8S71_RS12540 (H8S71_12535) rpsT 2458292..2458558 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  H8S71_RS12545 (H8S71_12540) holA 2458573..2459616 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  H8S71_RS12550 (H8S71_12545) - 2459656..2459769 (-) 114 WP_134975484.1 hypothetical protein -
  H8S71_RS12555 (H8S71_12550) yqzM 2459846..2459980 (+) 135 WP_003229983.1 YqzM family protein -
  H8S71_RS12560 (H8S71_12555) comEC 2460020..2462350 (-) 2331 WP_187001806.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  H8S71_RS12565 (H8S71_12560) comEB 2462354..2462923 (-) 570 WP_003229978.1 ComE operon protein 2 -
  H8S71_RS12570 (H8S71_12565) comEA 2462990..2463607 (-) 618 WP_163182169.1 competence protein ComEA Machinery gene
  H8S71_RS12575 (H8S71_12570) comER 2463691..2464512 (+) 822 WP_032726195.1 late competence protein ComER -
  H8S71_RS12580 (H8S71_12575) yqeM 2464578..2465321 (-) 744 WP_070547553.1 class I SAM-dependent methyltransferase -
  H8S71_RS12585 (H8S71_12580) rsfS 2465318..2465674 (-) 357 WP_014480315.1 ribosome silencing factor -
  H8S71_RS12590 (H8S71_12585) yqeK 2465692..2466252 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  H8S71_RS12595 (H8S71_12590) nadD 2466242..2466811 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  H8S71_RS12600 (H8S71_12595) yhbY 2466823..2467113 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 776 a.a.        Molecular weight: 86697.13 Da        Isoelectric Point: 6.9842

>NTDB_id=475901 H8S71_RS12560 WP_187001806.1 2460020..2462350(-) (comEC) [Bacillus subtilis subsp. subtilis strain ONU 559]
MRNSRLLLPMAAASATAGITAAAYFPAIFLFILFLLIMLIKTRHAFLIIVCFFSFILFFVLYAVTDSQNVSSYRQGTYQF
KAVIDTIPKIDGDRMSMMVETPDKEKWAAAYRIQSAGEKEQLLYIEPGMSCELTGTLEEPNHATVPGAFDYNEYLYRQHI
HWNYSVTSIQNCSEPENFKYKVLSLRKHIISFTNSLLPPDSAGIVQALTVGDRFYVEDEVLTAYQKLGVVHLLAISGLHV
GILTAGLFYIMIRLGITREKASILLLLFLPLYVMLTGAAPSVLRAALMSGVYLAGSLVKWRVHSATAICLSYIVLLLFNP
YHLFEAGFQLSFAVSFSLILSSSIFHQVKTSLGQLTIVSLIAQLGSLPILLYHFHQFSIISVPMNMLMVPFYTFCILPGA
VAGVLLLSLSASFGRLFFSWFDLVISWTNRLITNIADVDVFTIMIAHPAPVLLFLFTVTIILLLMAIEKRSLSQLIVTGG
ICCTVLFLLFIYPCLSSEGEVDMIDIGQGDSMFVGAPHQRGRVLIDTGGTLSYSSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGETETLLKHHKVKRLVIPKGFVSEPKDEKVLQTAREEGVTIEEVKRGDVLQIKDLQFHVLSPE
APDPASKNNSSLVLWMETGGMSWILTGDLEKEGEQEVMDVFPNIKADVLKVGHHGSKGSTGEEFIQQLQPKTAIISAGKN
NRYHHPHQEVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYPPYDTSDITETN

Nucleotide


Download         Length: 2331 bp        

>NTDB_id=475901 H8S71_RS12560 WP_187001806.1 2460020..2462350(-) (comEC) [Bacillus subtilis subsp. subtilis strain ONU 559]
ATGCGTAATTCGCGTTTGTTATTGCCTATGGCGGCAGCTTCGGCAACGGCTGGAATTACTGCCGCCGCTTATTTCCCTGC
TATTTTTCTTTTCATCCTCTTTCTCCTCATCATGTTAATCAAAACGAGGCACGCTTTTCTTATTATTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTGTACTGTATGCAGTCACAGATTCTCAGAATGTCTCTTCCTATCGGCAGGGAACCTATCAATTC
AAGGCAGTGATTGACACTATTCCCAAAATTGACGGCGACCGTATGTCTATGATGGTTGAGACACCTGATAAGGAAAAATG
GGCTGCTGCGTATCGCATTCAGTCTGCTGGTGAAAAAGAACAGCTGTTATACATAGAACCAGGAATGTCATGTGAGTTGA
CTGGTACATTGGAAGAACCGAATCACGCAACTGTGCCGGGTGCATTTGATTATAACGAGTATCTTTATCGGCAGCATATT
CATTGGAACTACTCTGTCACGTCTATCCAAAACTGCAGCGAACCTGAAAATTTTAAGTACAAGGTGCTCAGCTTGAGAAA
ACATATCATATCATTCACAAACAGCCTTCTGCCTCCTGATTCGGCAGGAATTGTACAGGCACTTACAGTCGGTGACAGAT
TTTATGTGGAGGACGAAGTGCTTACCGCGTATCAAAAGCTTGGTGTTGTCCATCTCTTGGCGATATCAGGACTCCACGTG
GGGATTTTGACAGCAGGTTTGTTTTATATCATGATCCGTCTTGGTATAACTAGAGAAAAGGCGTCAATTCTGTTGCTGTT
ATTTCTGCCGCTCTATGTGATGTTGACCGGCGCTGCTCCTTCAGTGCTACGCGCCGCTCTCATGTCGGGTGTTTACTTAG
CTGGAAGCCTTGTCAAATGGCGTGTCCACTCTGCAACTGCAATTTGTCTTTCATACATCGTCCTTCTGCTCTTCAATCCT
TATCATCTCTTTGAAGCCGGTTTTCAGCTATCATTCGCCGTCAGTTTTTCTTTAATTCTATCCTCTTCTATTTTTCATCA
GGTTAAAACCTCCTTGGGGCAGCTGACAATTGTATCACTCATCGCTCAGCTGGGCTCGCTTCCGATTCTCCTATATCATT
TTCATCAGTTTTCTATCATCAGCGTACCGATGAATATGTTGATGGTACCATTTTATACCTTCTGTATTTTGCCGGGAGCT
GTAGCAGGTGTTCTTCTATTAAGTCTTTCCGCTTCGTTTGGGAGATTGTTTTTCAGCTGGTTTGATTTAGTGATAAGCTG
GACCAATAGGCTAATCACAAACATTGCAGATGTTGATGTGTTCACGATTATGATCGCACATCCTGCACCTGTTTTGCTTT
TTTTATTCACGGTCACGATCATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTGTCGCAGTTGATAGTAACCGGAGGC
ATTTGCTGCACGGTGCTGTTTCTGCTCTTTATATATCCGTGTCTTAGTTCCGAAGGAGAAGTGGATATGATAGATATTGG
ACAGGGTGACAGCATGTTTGTAGGTGCTCCGCATCAGCGGGGTCGTGTCTTAATTGATACCGGCGGCACTTTGTCTTACT
CGTCAGAGCCTTGGCGCGAAAAACAGCATCCGTTTTCACTGGGGGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATCAAACAGCTTGACGCTTTAATTCTGACGCACGCTGACCAAGATCATATCGGAGAGACGGAGACTCTGCTGAAGCATCA
TAAAGTAAAGCGCCTCGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGATGAGAAAGTGCTGCAGACAGCCAGAGAAG
AGGGAGTGACAATTGAAGAGGTGAAGCGAGGCGATGTATTGCAAATAAAGGATTTGCAGTTCCATGTACTGTCACCTGAA
GCACCTGATCCGGCAAGCAAAAATAATTCCTCTCTCGTTCTGTGGATGGAGACGGGCGGTATGAGCTGGATTTTGACGGG
TGATCTGGAGAAAGAAGGGGAACAGGAGGTGATGGACGTGTTTCCAAATATTAAAGCAGATGTCTTAAAGGTGGGGCACC
ATGGGAGCAAAGGCTCTACCGGTGAAGAATTCATCCAACAGCTTCAGCCGAAAACGGCCATTATCTCAGCCGGAAAAAAC
AATCGGTACCATCATCCTCATCAAGAAGTTCTGCAACTATTACAGAGACATTCTATCCGCGTGCTGCGAACAGATCAAAA
CGGAACGATCCAATATAGATACAAAAACAGAGTTGGAACCTTTTCTGTCTATCCTCCATATGATACATCAGATATAACAG
AGACGAACTAA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-469)

Domain of unknown function (DUF4131) (DUF4131)

(19-170)

Metallo-beta-lactamase superfamily (Lactamase_B)

(507-718)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

98.196

100

0.982