Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7683_RS21250 Genome accession   NZ_CP060288
Coordinates   4538787..4539995 (+) Length   402 a.a.
NCBI ID   WP_209449787.1    Uniprot ID   -
Organism   Pseudomonas mendocina strain CPS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4533787..4544995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7683_RS21215 (H7683_21195) nfsB 4533798..4534448 (+) 651 WP_209449784.1 oxygen-insensitive NAD(P)H nitroreductase -
  H7683_RS21220 (H7683_21200) - 4534677..4535072 (-) 396 WP_115297756.1 RidA family protein -
  H7683_RS21225 (H7683_21205) - 4535213..4536133 (+) 921 WP_209449785.1 LysR family transcriptional regulator -
  H7683_RS21230 (H7683_21210) - 4536293..4536883 (+) 591 WP_017362970.1 TetR/AcrR family transcriptional regulator -
  H7683_RS21235 (H7683_21215) - 4536893..4537372 (+) 480 WP_017362971.1 nuclear transport factor 2 family protein -
  H7683_RS21240 (H7683_21220) - 4537441..4538352 (+) 912 WP_209449786.1 LysR family transcriptional regulator -
  H7683_RS21245 (H7683_21225) - 4538360..4538638 (-) 279 WP_013715799.1 peptidylprolyl isomerase -
  H7683_RS21250 (H7683_21230) pilU 4538787..4539995 (+) 1209 WP_209449787.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7683_RS21255 (H7683_21235) - 4540177..4540926 (-) 750 WP_115291450.1 transporter substrate-binding domain-containing protein -
  H7683_RS21260 (H7683_21240) - 4540995..4542950 (-) 1956 WP_209449788.1 acetoacetate--CoA ligase -
  H7683_RS21265 (H7683_21245) - 4543180..4544082 (-) 903 WP_209449789.1 hydroxymethylglutaryl-CoA lyase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 44361.59 Da        Isoelectric Point: 6.0784

>NTDB_id=475390 H7683_RS21250 WP_209449787.1 4538787..4539995(+) (pilU) [Pseudomonas mendocina strain CPS5]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSQEPMKAGDVAAIADSIMDSAQREEFERELEMNLAISLAGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLYRDNPANTRPAPAAAAPVAAPKAASAGDWGLELKLEDIEEEQPPEDPGRQ
GI

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=475390 H7683_RS21250 WP_209449787.1 4538787..4539995(+) (pilU) [Pseudomonas mendocina strain CPS5]
ATGGATCTCCACGCAATGCTGAAAATCCTGTCCAGTCAGGATGGCTCCGATCTGTATCTGTCCACCGGCGCGCCGCCTTG
CGCCAAGTTCAACGGCGTGCTCAAGGCGCTGAGCCAGGAGCCGATGAAGGCGGGCGATGTGGCGGCCATTGCTGATTCGA
TCATGGATAGCGCGCAGCGCGAGGAGTTCGAGCGCGAACTGGAGATGAACCTGGCGATTTCTCTGGCTGGCGTGGGGCGT
TTTCGCATCAACATCTTCAAGCAGCGCAACGAGGTATCCATCGTCGCGCGTAACATCAAGCTGGACATCCCCCGCTTTGA
AGACCTCAAGCTGCCCGAGGTGCTGCTCAGCACGGTGATGGAAAAGCGCGGCCTGGTGTTGTTCGTTGGCGGCACCGGCT
CTGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTGGGCGTGGACACGCGCAGCTTCCATGC
CGCGCTGAAGAACACCTTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCGACCGCGAAACCATGGAGCATG
CCCTGGCCTTTGCTGATACCGGGCACCTGGCGATCTCCACTTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATT
ATCAACTTCTTTCCGGAAGACCGCCGCCCTCAGTTGCTCAACGACCTGGGTAACAACCTCAAGGCGTTCGTTTCCCAGCG
CCTGGTCAAGACCGTCGATGGCAAGCGCCGCGCGGCAGTGGAGGTGATGCTGGGTACGCCGACGGTGCGCGACCTGATCA
AACGCAACGAATTCTCCGAGCTCAAGGAGATCATGGAGAAATCCAAGAACCTGGGCATGCAGACCTTCGACCAGGCGCTG
ATCGATCTGGTGCACGAAGGCGCAATCGATGAAGAAGAAGCGGTGAAGAACGCCGACTCGGCGAACAACGTGCGTCTGAA
ACTCAAGCTCTATCGCGACAATCCGGCCAATACCAGGCCTGCACCCGCCGCGGCAGCGCCGGTCGCTGCACCGAAGGCCG
CCTCGGCAGGCGATTGGGGTCTGGAGCTCAAGCTGGAGGATATCGAGGAGGAGCAGCCACCGGAGGACCCGGGACGGCAG
GGGATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.41

87.313

0.493
  pilU Acinetobacter baylyi ADP1

51.989

87.562

0.455
  pilU Vibrio cholerae strain A1552

51.558

87.811

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.075

96.766

0.378