Detailed information NTG0474496

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: H7F27_RS08210
• Genome accession: NZ_CP060193
• Coordinates: 1624572..1625189 (+)
• Length: 205 a.a.
• NCBI ID: WP_185848809.1
• Uniprot ID: A0A7G7UHK2
• Organism: Bacillus sp. PAMC26543
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1619572..1630189

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7F27_RS08175 (H7F27_08175)	aroE	1620232..1621068 (+)	837	WP_038954228.1	shikimate dehydrogenase	-
H7F27_RS08180 (H7F27_08180)	yhbY	1621068..1621358 (+)	291	WP_024122118.1	ribosome assembly RNA-binding protein YhbY	-
H7F27_RS08185 (H7F27_08185)	-	1621369..1621938 (+)	570	WP_010335014.1	nicotinate-nucleotide adenylyltransferase	-
H7F27_RS08190 (H7F27_08190)	yqeK	1621928..1622488 (+)	561	WP_185848808.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
H7F27_RS08195 (H7F27_08195)	rsfS	1622506..1622862 (+)	357	WP_044154484.1	ribosome silencing factor	-
H7F27_RS08200 (H7F27_08200)	-	1622859..1623602 (+)	744	WP_024122114.1	class I SAM-dependent methyltransferase	-
H7F27_RS08205 (H7F27_08205)	comER	1623667..1624488 (-)	822	WP_024122113.1	late competence protein ComER	-
H7F27_RS08210 (H7F27_08210)	comEA	1624572..1625189 (+)	618	WP_185848809.1	helix-hairpin-helix domain-containing protein	Machinery gene
H7F27_RS08215 (H7F27_08215)	-	1625256..1625825 (+)	570	WP_010335007.1	ComE operon protein 2	-
H7F27_RS08220 (H7F27_08220)	-	1625829..1628148 (+)	2320	Protein_1610	DNA internalization-related competence protein ComEC/Rec2	-
H7F27_RS08225 (H7F27_08225)	-	1628187..1628321 (-)	135	WP_010335005.1	YqzM family protein	-
H7F27_RS08230 (H7F27_08230)	-	1628363..1628512 (+)	150	WP_003229985.1	hypothetical protein	-
H7F27_RS08235 (H7F27_08235)	holA	1628552..1629595 (+)	1044	WP_024122110.1	DNA polymerase III subunit delta	-
H7F27_RS08240 (H7F27_08240)	rpsT	1629610..1629876 (-)	267	WP_010335003.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 22030.88 Da
• Isoelectric Point: 4.7586

>NTDB_id=474496 H7F27_RS08210 WP_185848809.1 1624572..1625189(+) (comEA) [Bacillus sp. PAMC26543]
MNWLNQHKTIIMSVGSAAVLIAVLCLFFFSGNNKEPVKQVVSSEKENEEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSKGEEAAQQAAGGAVQSSTGKETLVNINIATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=474496 H7F27_RS08210 WP_185848809.1 1624572..1625189(+) (comEA) [Bacillus sp. PAMC26543]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGTTGGCTCTGCTGCTGTTCTCATTGCAGTTCTTTGCCTCTT
TTTTTTCAGCGGAAATAACAAAGAGCCTGTAAAACAAGTTGTATCTAGTGAAAAGGAAAATGAGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCGGAAGAAAATATTGTAATCGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGCGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTATATACCGAGTAAGGGAGAAGAGGCGGCACAGCAAGCGGCCGGCGGTGCTG
TACAAAGCAGTACGGGGAAGGAGACTTTGGTCAATATCAACATAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGGCCTTCAAAAGCCGAAGCGATTATTGCGTATCGGGAAGAGAACGGGCGTTTCCAGACAATCGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-203)

• SLBB domain (SLBB) (64-119)

3D structure

• AlphaFold DB: A0A7G7UHK2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	76.699	100	0.771
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.937	100	0.4
comEA	Latilactobacillus sakei subsp. sakei 23K	36.239	100	0.385
comEA	Streptococcus thermophilus LMD-9	36.792	100	0.38