Detailed information NTG0474370

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JT750_RS29310
• Genome accession: NZ_CP070471
• Coordinates: 6368472..6369620 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain B17932
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6363472..6374620

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JT750_RS29290 (JT750_29290)	-	6364209..6365393 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
JT750_RS29295 (JT750_29295)	-	6365390..6366763 (-)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
JT750_RS29300 (JT750_29300)	-	6367006..6367410 (-)	405	WP_003084560.1	NINE protein	-
JT750_RS29305 (JT750_29305)	czcD	6367565..6368464 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JT750_RS29310 (JT750_29310)	pilU	6368472..6369620 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
JT750_RS29315 (JT750_29315)	pilT	6369798..6370832 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JT750_RS29320 (JT750_29320)	-	6371047..6371739 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
JT750_RS29325 (JT750_29325)	proC	6371751..6372572 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
JT750_RS29330 (JT750_29330)	-	6372583..6373176 (+)	594	WP_003084544.1	YggT family protein	-
JT750_RS29335 (JT750_29335)	-	6373173..6373469 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=474370 JT750_RS29310 WP_003110016.1 6368472..6369620(-) (pilU) [Pseudomonas aeruginosa strain B17932]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=474370 JT750_RS29310 WP_003110016.1 6368472..6369620(-) (pilU) [Pseudomonas aeruginosa strain B17932]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382