Detailed information NTG0474283

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JT749_RS30405
• Genome accession: NZ_CP070467
• Coordinates: 6607112..6608260 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain B17416
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6602112..6613260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JT749_RS30385 (JT749_30385)	-	6602849..6604033 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
JT749_RS30390 (JT749_30390)	-	6604030..6605403 (-)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
JT749_RS30395 (JT749_30395)	-	6605646..6606050 (-)	405	WP_003084560.1	NINE protein	-
JT749_RS30400 (JT749_30400)	czcD	6606205..6607104 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JT749_RS30405 (JT749_30405)	pilU	6607112..6608260 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
JT749_RS30410 (JT749_30410)	pilT	6608438..6609472 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JT749_RS30415 (JT749_30415)	-	6609687..6610379 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
JT749_RS30420 (JT749_30420)	proC	6610391..6611212 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
JT749_RS30425 (JT749_30425)	-	6611223..6611816 (+)	594	WP_003084544.1	YggT family protein	-
JT749_RS30430 (JT749_30430)	-	6611813..6612109 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=474283 JT749_RS30405 WP_003110016.1 6607112..6608260(-) (pilU) [Pseudomonas aeruginosa strain B17416]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=474283 JT749_RS30405 WP_003110016.1 6607112..6608260(-) (pilU) [Pseudomonas aeruginosa strain B17416]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATAACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382