Detailed information NTG0474216

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: XFGV230_RS05875
• Genome accession: NZ_CP060159
• Coordinates: 1322628..1323758 (+)
• Length: 376 a.a.
• NCBI ID: WP_011097971.1
• Uniprot ID: Q87CD3
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1317628..1328758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XFGV230_RS05855 (XFGV230_05855)	-	1317915..1318739 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
XFGV230_RS05860 (XFGV230_05860)	-	1319373..1320200 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
XFGV230_RS05865 (XFGV230_05865)	-	1320558..1320770 (-)	213	WP_004087301.1	hypothetical protein	-
XFGV230_RS05870 (XFGV230_05870)	pilT	1321428..1322462 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
XFGV230_RS05875 (XFGV230_05875)	pilU	1322628..1323758 (+)	1131	WP_011097971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
XFGV230_RS05880 (XFGV230_05880)	-	1324451..1325419 (-)	969	WP_004087304.1	YafY family protein	-
XFGV230_RS05885 (XFGV230_05885)	-	1326268..1327077 (+)	810	WP_004087308.1	DUF481 domain-containing protein	-
XFGV230_RS05890 (XFGV230_05890)	hemC	1327131..1328048 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42479.73 Da
• Isoelectric Point: 6.5563

>NTDB_id=474216 XFGV230_RS05875 WP_011097971.1 1322628..1323758(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain GV230]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=474216 XFGV230_RS05875 WP_011097971.1 1322628..1323758(+) (pilU) [Xylella fastidiosa subsp. fastidiosa strain GV230]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: Q87CD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402