Detailed information NTG0473427

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H4B47_RS01140
• Genome accession: NZ_CP060029
• Coordinates: 229726..230844 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AbCTX1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 224726..235844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H4B47_RS01130 (H4B47_01135)	-	227842..228534 (-)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-
H4B47_RS01135 (H4B47_01140)	pilT	228661..229698 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H4B47_RS01140 (H4B47_01145)	pilU	229726..230844 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H4B47_RS01145 (H4B47_01150)	fur	230914..231351 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H4B47_RS01150 (H4B47_01155)	-	231463..231861 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H4B47_RS01155 (H4B47_01160)	-	231916..232239 (-)	324	WP_000442585.1	RnfH family protein	-
H4B47_RS01160 (H4B47_01165)	-	232239..233306 (-)	1068	WP_029776981.1	membrane protein	-
H4B47_RS01165 (H4B47_01170)	-	233459..233926 (-)	468	WP_203469506.1	bacteriohemerythrin	-
H4B47_RS01170 (H4B47_01175)	-	234184..235032 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=473427 H4B47_RS01140 WP_000347036.1 229726..230844(+) (pilU) [Acinetobacter baumannii strain AbCTX1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=473427 H4B47_RS01140 WP_000347036.1 229726..230844(+) (pilU) [Acinetobacter baumannii strain AbCTX1]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392