Detailed information NTG0473321

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H5029_RS14035
• Genome accession: NZ_CP060011
• Coordinates: 2963791..2964909 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain TP2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2958791..2969909

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H5029_RS14025 (H5029_14020)	-	2961907..2962599 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
H5029_RS14030 (H5029_14025)	pilT	2962726..2963763 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H5029_RS14035 (H5029_14030)	pilU	2963791..2964909 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H5029_RS14040 (H5029_14035)	fur	2964979..2965416 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H5029_RS14045 (H5029_14040)	-	2965528..2965926 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H5029_RS14050 (H5029_14045)	-	2965981..2966304 (-)	324	WP_000442587.1	RnfH family protein	-
H5029_RS14055 (H5029_14050)	-	2966304..2967371 (-)	1068	WP_002019572.1	hypothetical protein	-
H5029_RS14060 (H5029_14055)	-	2967524..2967979 (-)	456	WP_000782976.1	bacteriohemerythrin	-
H5029_RS14065 (H5029_14060)	-	2968232..2969080 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=473321 H5029_RS14035 WP_000347039.1 2963791..2964909(+) (pilU) [Acinetobacter baumannii strain TP2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=473321 H5029_RS14035 WP_000347039.1 2963791..2964909(+) (pilU) [Acinetobacter baumannii strain TP2]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395