Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H4P35_RS00620 Genome accession   NZ_CP059848
Coordinates   133544..134989 (+) Length   481 a.a.
NCBI ID   WP_056327751.1    Uniprot ID   -
Organism   Achromobacter sp. 77     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 128544..139989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H4P35_RS00605 (H4P35_00605) - 129066..130394 (+) 1329 WP_180099774.1 amidase -
  H4P35_RS00610 (H4P35_00610) leuA 130655..132364 (-) 1710 WP_062840150.1 2-isopropylmalate synthase -
  H4P35_RS00615 (H4P35_00615) - 132883..133545 (-) 663 WP_180099770.1 hypothetical protein -
  H4P35_RS00620 (H4P35_00620) rpoN/rpoN1 133544..134989 (+) 1446 WP_056327751.1 RNA polymerase factor sigma-54 Machinery gene
  H4P35_RS00625 (H4P35_00625) - 135111..135389 (+) 279 WP_056327752.1 bacterioferritin-associated ferredoxin -
  H4P35_RS00630 (H4P35_00630) - 135419..136282 (-) 864 WP_226847690.1 LysR substrate-binding domain-containing protein -
  H4P35_RS00635 (H4P35_00635) - 136415..137326 (+) 912 WP_226847691.1 DMT family transporter -
  H4P35_RS00640 (H4P35_00640) bfr 137718..138194 (+) 477 WP_056327755.1 bacterioferritin -
  H4P35_RS00645 (H4P35_00645) - 138288..138962 (-) 675 WP_226847692.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52645.98 Da        Isoelectric Point: 4.7656

>NTDB_id=472205 H4P35_RS00620 WP_056327751.1 133544..134989(+) (rpoN/rpoN1) [Achromobacter sp. 77]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPATAEDRAESERESSVQDDEP
AAERETAIDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDAALASLLIDELDENGYLGSPLDEILGWLP
AEMEPDMDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDLTLLPEAADPVVLACARRICAEHLPLLATGNAARLCAA
VGCDETTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=472205 H4P35_RS00620 WP_056327751.1 133544..134989(+) (rpoN/rpoN1) [Achromobacter sp. 77]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAGAATCCCCTGC
TGGATCGCGAGGACGAGCCGGCGACGGCGGAGGATCGGGCCGAATCCGAGCGCGAATCTTCGGTTCAGGACGACGAACCG
GCGGCCGAGCGCGAGACCGCCATCGACGAGATGCCTGGCTCGGGCGGGGTGTATCCCGACGACGACGCCAGCCTGCCCGA
GGCGGCGCGGCCGGACACGCTGCGCGAACATCTGCTGGGTCAGCTTGCCCTGACGCGCGCCGCGCCGCGCGATGCCGCGC
TGGCGTCCCTGTTGATCGACGAACTGGACGAAAACGGCTACCTGGGTTCGCCGCTGGACGAAATCCTGGGTTGGCTCCCT
GCCGAAATGGAGCCCGACATGGACGAGCTGCGCGCCGCCCTGTCGCTTCTGCAATCGTTCGACCCGGTGGGCATCGGCGC
GCGCGACATGGCTGAATGCCTGCAACTGCAACTGCGCAATCCCGATCTCACCTTGCTGCCGGAGGCGGCTGACCCCGTCG
TGCTGGCCTGTGCGCGCCGGATCTGCGCCGAGCACCTGCCGCTGTTGGCAACCGGCAACGCCGCGCGCCTGTGTGCGGCC
GTCGGCTGCGACGAGACCACGTTCCGCGCGGCGCATTCGCTGATCTTGCGCCTGGAACCCCGGCCGGGTCGGGCCTGGAC
CGTGCCGGCGGCCGAGTACGCAGTGCCGGACGTCATCGTCCGCAAGACGCGGCGCGGCTGGCAGGCCACGCTGAATAGCG
CCGCGGTGCCGCGGCTGCAGATCAATGGGCTGTACGCGCAGATGCTGGGCAACCAGAAGGAATCCGCCCACGCCGGCCTT
CAGGCCCAGCTGCAGCAGGCCAAATGGATGATCCGCAACGTCGAGCAGCGCTTTGACACGATCCTGCGCGTGTCGCAGGC
CATCGTCGAGCGTCAGACGGCTTTCTTCAGCCAGGGGCCGGCGGCGATGCGTCCGCTCATCCTGAAAGACATCGCCGGCG
AGCTGGGTTTGCATGAATCGACCATTTCCCGGGCCACAACACAGAAGTACATGCTCACTCCATTCGGCACGCTGGAGCTC
AAGCGCTTCTTTGGAACGGGCGTGTCGACAGAAGGCGGCGACGCCACGTCGGCTACCGCGGTGCAGACCTACATCCGCCA
GATGGTGGCCGACGAGAACCGCGCCAAGCCGTTGTCGGATAGCCAGATCATGCAAAAACTGGCCGATCAGGGGATAGTGA
TTGCGCGCCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCCGCCGCCCTGCGCAAGGCGCAGGCCTCCGCA
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.907

100

0.511