Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   H2O77_RS05200 Genome accession   NZ_CP059843
Coordinates   1226300..1226845 (-) Length   181 a.a.
NCBI ID   WP_215824073.1    Uniprot ID   -
Organism   Cobetia sp. 4B     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1215204..1263674 1226300..1226845 within 0


Gene organization within MGE regions


Location: 1215204..1263674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2O77_RS05145 (H2O77_05145) - 1215204..1216127 (-) 924 WP_054554924.1 LysR family transcriptional regulator -
  H2O77_RS05150 (H2O77_05150) trpB 1216362..1217603 (+) 1242 WP_215824065.1 tryptophan synthase subunit beta -
  H2O77_RS05155 (H2O77_05155) trpA 1217730..1218569 (+) 840 WP_107335249.1 tryptophan synthase subunit alpha -
  H2O77_RS05160 (H2O77_05160) - 1218739..1219524 (-) 786 WP_215824066.1 alpha/beta fold hydrolase -
  H2O77_RS05165 (H2O77_05165) - 1220075..1222426 (+) 2352 WP_215824067.1 EAL domain-containing protein -
  H2O77_RS05175 (H2O77_05175) - 1222856..1224100 (+) 1245 WP_215824068.1 tyrosine-type recombinase/integrase -
  H2O77_RS05180 (H2O77_05180) - 1224069..1224302 (-) 234 WP_215824069.1 AlpA family phage regulatory protein -
  H2O77_RS05185 (H2O77_05185) - 1224316..1224465 (-) 150 WP_215824070.1 hypothetical protein -
  H2O77_RS05190 (H2O77_05190) - 1224650..1225564 (-) 915 WP_215824071.1 DNA cytosine methyltransferase -
  H2O77_RS05195 (H2O77_05195) - 1225561..1226190 (-) 630 WP_215824072.1 hypothetical protein -
  H2O77_RS05200 (H2O77_05200) ssb 1226300..1226845 (-) 546 WP_215824073.1 single-stranded DNA-binding protein Machinery gene
  H2O77_RS05205 (H2O77_05205) - 1227221..1227748 (+) 528 WP_215824074.1 hypothetical protein -
  H2O77_RS05210 (H2O77_05210) - 1228104..1228430 (-) 327 WP_215824075.1 hypothetical protein -
  H2O77_RS05215 (H2O77_05215) - 1228735..1229610 (-) 876 WP_240548720.1 helix-turn-helix transcriptional regulator -
  H2O77_RS05220 (H2O77_05220) - 1229533..1229751 (+) 219 WP_215824077.1 Cro/CI family transcriptional regulator -
  H2O77_RS05225 (H2O77_05225) - 1229814..1230308 (+) 495 WP_215824078.1 phage regulatory CII family protein -
  H2O77_RS05230 (H2O77_05230) - 1230391..1230717 (+) 327 WP_215824079.1 hypothetical protein -
  H2O77_RS05235 (H2O77_05235) - 1231049..1231861 (+) 813 WP_215824080.1 DnaT-like ssDNA-binding domain-containing protein -
  H2O77_RS05240 (H2O77_05240) - 1231800..1232543 (+) 744 WP_215824081.1 replication protein P -
  H2O77_RS05245 (H2O77_05245) - 1232536..1232892 (+) 357 WP_215824082.1 hypothetical protein -
  H2O77_RS05250 (H2O77_05250) - 1233074..1233358 (+) 285 WP_176676551.1 hypothetical protein -
  H2O77_RS05255 (H2O77_05255) - 1233355..1233891 (+) 537 WP_240548721.1 VRR-NUC domain-containing protein -
  H2O77_RS05260 (H2O77_05260) - 1233914..1234474 (+) 561 WP_215824083.1 hypothetical protein -
  H2O77_RS05265 (H2O77_05265) - 1235198..1235728 (+) 531 WP_215824084.1 glycoside hydrolase family 104 protein -
  H2O77_RS05270 (H2O77_05270) - 1235773..1236126 (+) 354 WP_131431534.1 phage holin, lambda family -
  H2O77_RS05275 (H2O77_05275) - 1236114..1236539 (+) 426 WP_175089174.1 hypothetical protein -
  H2O77_RS05280 (H2O77_05280) - 1236523..1236750 (+) 228 WP_215824085.1 hypothetical protein -
  H2O77_RS05285 (H2O77_05285) - 1236930..1237505 (+) 576 WP_215824086.1 KilA-N domain-containing protein -
  H2O77_RS05290 (H2O77_05290) - 1237523..1237714 (-) 192 WP_215824087.1 DUF2188 domain-containing protein -
  H2O77_RS05300 (H2O77_05300) - 1238099..1238437 (+) 339 WP_240548722.1 HNH endonuclease -
  H2O77_RS05305 (H2O77_05305) - 1238550..1239029 (+) 480 WP_215824089.1 phage terminase small subunit P27 family -
  H2O77_RS05310 (H2O77_05310) - 1239036..1240745 (+) 1710 WP_166019414.1 terminase TerL endonuclease subunit -
  H2O77_RS05315 (H2O77_05315) - 1240747..1240911 (+) 165 WP_166019415.1 hypothetical protein -
  H2O77_RS05320 (H2O77_05320) - 1240908..1242128 (+) 1221 WP_215824090.1 phage portal protein -
  H2O77_RS05325 (H2O77_05325) - 1242125..1242727 (+) 603 WP_084208182.1 HK97 family phage prohead protease -
  H2O77_RS05330 (H2O77_05330) - 1242743..1243978 (+) 1236 WP_215824091.1 phage major capsid protein -
  H2O77_RS05335 (H2O77_05335) - 1244056..1244385 (+) 330 WP_215824092.1 head-tail connector protein -
  H2O77_RS05340 (H2O77_05340) - 1244393..1244938 (+) 546 WP_215824093.1 head-tail adaptor protein -
  H2O77_RS05345 (H2O77_05345) - 1244950..1245528 (+) 579 WP_215824094.1 phage tail protein -
  H2O77_RS05350 (H2O77_05350) - 1245525..1245965 (+) 441 WP_215824095.1 hypothetical protein -
  H2O77_RS05355 (H2O77_05355) - 1245969..1246715 (+) 747 WP_215824096.1 hypothetical protein -
  H2O77_RS05360 (H2O77_05360) - 1246728..1246901 (+) 174 WP_215824097.1 hypothetical protein -
  H2O77_RS05365 (H2O77_05365) - 1246973..1250776 (+) 3804 WP_215824098.1 hypothetical protein -
  H2O77_RS05370 (H2O77_05370) - 1250810..1251208 (+) 399 WP_215824099.1 hypothetical protein -
  H2O77_RS05375 (H2O77_05375) - 1251237..1254611 (+) 3375 WP_215824100.1 hypothetical protein -
  H2O77_RS05380 (H2O77_05380) - 1254692..1255849 (+) 1158 WP_215824101.1 hypothetical protein -
  H2O77_RS05385 (H2O77_05385) - 1255850..1257535 (+) 1686 WP_215824102.1 LamG-like jellyroll fold domain-containing protein -
  H2O77_RS05390 (H2O77_05390) - 1257532..1257933 (+) 402 WP_215824103.1 hypothetical protein -
  H2O77_RS05395 (H2O77_05395) - 1257935..1259803 (+) 1869 WP_215824104.1 hypothetical protein -
  H2O77_RS05400 (H2O77_05400) - 1259846..1260067 (+) 222 WP_215824105.1 hypothetical protein -
  H2O77_RS05405 (H2O77_05405) - 1260129..1262654 (-) 2526 WP_215824106.1 S8 family peptidase -
  H2O77_RS05410 (H2O77_05410) - 1262685..1263674 (-) 990 WP_215824107.1 ATP-binding protein -

Sequence


Protein


Download         Length: 181 a.a.        Molecular weight: 19925.98 Da        Isoelectric Point: 6.8812

>NTDB_id=472190 H2O77_RS05200 WP_215824073.1 1226300..1226845(-) (ssb) [Cobetia sp. 4B]
MARGINKAILIGHLGQDPEVRFTPSGSAVANLSIATTDKWRDRQTGNVQERTEWHRVVMFNKTAEIAQQYLKKGAQVYIE
GRLQTRKWTDQQGIERYSTEIVANDMQMLGGSGGGQPSQAQRPPAQQNYQQPPQQQGGYQQPAPASTHHGVGQPPAGQQQ
PNNFGAPPAGSFDDFDDEIPF

Nucleotide


Download         Length: 546 bp        

>NTDB_id=472190 H2O77_RS05200 WP_215824073.1 1226300..1226845(-) (ssb) [Cobetia sp. 4B]
ATGGCCCGCGGCATCAACAAGGCGATCCTGATCGGCCATCTCGGCCAAGACCCAGAGGTGCGCTTCACCCCCAGCGGCTC
AGCCGTGGCCAACCTCAGCATCGCCACCACCGACAAGTGGCGAGACAGGCAGACCGGCAACGTCCAAGAACGCACCGAAT
GGCATCGGGTGGTGATGTTCAACAAGACCGCCGAGATCGCTCAGCAGTACCTCAAGAAAGGCGCTCAGGTGTATATCGAA
GGACGCTTGCAGACTCGCAAGTGGACGGACCAGCAAGGCATCGAACGCTACAGCACCGAGATCGTCGCCAATGACATGCA
GATGCTCGGCGGTTCCGGTGGCGGTCAGCCATCCCAGGCACAGCGTCCGCCAGCTCAGCAGAACTATCAGCAGCCACCCC
AGCAGCAAGGCGGCTATCAGCAACCGGCGCCCGCCAGCACGCACCACGGTGTAGGTCAGCCACCTGCAGGCCAACAACAG
CCGAACAACTTCGGGGCTCCGCCAGCCGGCAGCTTCGATGACTTTGATGACGAGATCCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

52.406

100

0.541

  ssb Neisseria gonorrhoeae MS11

50

99.448

0.497

  ssb Neisseria meningitidis MC58

49.444

99.448

0.492

  ssb Glaesserella parasuis strain SC1401

46.237

100

0.475