Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H3303_RS14445 Genome accession   NZ_CP059738
Coordinates   3244923..3246032 (+) Length   369 a.a.
NCBI ID   WP_016984335.1    Uniprot ID   A0A7H8GLV1
Organism   Pseudomonas sp. 29A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3239923..3251032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3303_RS14430 (H3303_14430) - 3240883..3241821 (+) 939 WP_175554474.1 sugar kinase -
  H3303_RS14435 (H3303_14435) - 3241930..3242205 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  H3303_RS14440 (H3303_14440) - 3242257..3244794 (-) 2538 WP_175554473.1 PAS domain-containing sensor histidine kinase -
  H3303_RS14445 (H3303_14445) pilU 3244923..3246032 (+) 1110 WP_016984335.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H3303_RS14450 (H3303_14450) - 3246233..3249934 (+) 3702 WP_175554472.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41008.02 Da        Isoelectric Point: 6.7289

>NTDB_id=471685 H3303_RS14445 WP_016984335.1 3244923..3246032(+) (pilU) [Pseudomonas sp. 29A]
MEIDALLKQLASRHGSDLYLSTGAPPSARFEGVLTPLSEQFFKPGEVAMIAASLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLDTVMLKQGLILFVGATDCGKSTSLAALIDHRNRHSSGHIVTIED
PIEYIHQHRLSIINQREVGVDTRSFQAALKNTLRQAPDVVLIGEIRDRETMEHALSFAETGHLVLSTLHANNANQALDRI
INMFAEERRPQLLQALGNNLKACISQRLVRTMDGKRRAAVEVLLGSPTVADLIHRSELGELKKLMEKSSESGMQTFDTAL
FALVSEGVIAEEEAFKHADSASNLKLRMRLVGNTAVTEDSNSGKWGLIE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=471685 H3303_RS14445 WP_016984335.1 3244923..3246032(+) (pilU) [Pseudomonas sp. 29A]
ATGGAAATCGACGCACTGCTTAAACAACTGGCGAGCCGTCACGGCTCGGATCTCTATCTTTCCACAGGCGCGCCGCCAAG
TGCTCGGTTCGAAGGGGTGCTGACGCCGCTGAGCGAGCAATTCTTCAAACCCGGCGAAGTGGCGATGATTGCCGCTTCCC
TGATGGACGCCGAGCAACGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAACGGGCGTTGGCCGC
TTTCGCGTCAACATCTTCAAACAGCGCAACGATGTATCAATTGTAATTCGTAACGTCAAACTCGATATTCCGCGTTTCGA
GGACCTGAAGCTACCGCCGGTGTTGCTCGACACCGTGATGCTCAAACAAGGCTTGATCCTGTTCGTCGGCGCTACCGACT
GCGGCAAGTCGACGTCGTTGGCAGCACTGATCGATCACCGCAACCGCCATAGTAGCGGGCACATCGTCACTATCGAGGAT
CCGATTGAATACATCCATCAGCACAGGCTGTCGATCATCAATCAGCGTGAAGTCGGCGTCGACACGCGCAGCTTTCAAGC
TGCATTGAAAAACACCCTGCGCCAGGCCCCCGACGTCGTGCTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CGCTTTCGTTTGCCGAGACCGGGCATCTGGTTCTGTCGACATTGCACGCCAATAACGCCAATCAGGCGTTGGACCGGATT
ATCAATATGTTTGCCGAAGAGCGACGACCGCAGTTGTTGCAGGCGTTGGGCAACAATCTGAAAGCCTGTATCTCGCAGCG
ACTGGTGCGCACGATGGATGGGAAAAGAAGGGCGGCCGTCGAGGTGTTATTGGGGTCGCCTACCGTCGCTGATCTAATTC
ATCGGAGTGAACTTGGTGAACTAAAAAAACTGATGGAGAAGTCGTCTGAGTCGGGCATGCAAACTTTCGATACGGCGCTG
TTCGCCTTGGTGTCGGAGGGTGTAATTGCCGAAGAAGAAGCTTTCAAGCATGCAGACTCGGCGAGTAATCTGAAGCTACG
CATGAGGCTGGTCGGGAATACAGCGGTGACAGAAGATTCAAACTCGGGAAAGTGGGGTTTGATCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8GLV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.648

96.206

0.526
  pilU Acinetobacter baylyi ADP1

52.557

95.393

0.501
  pilU Vibrio cholerae strain A1552

49.725

98.645

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.181

92.683

0.363