Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H2513_RS10505 Genome accession   NZ_CP059704
Coordinates   2228025..2229359 (+) Length   444 a.a.
NCBI ID   WP_203331791.1    Uniprot ID   -
Organism   Pasteurella multocida strain RCAD0730     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2223025..2234359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2513_RS10480 (H2513_10460) - 2223101..2225662 (-) 2562 WP_316729874.1 penicillin-binding protein 1A -
  H2513_RS10485 (H2513_10465) - 2225798..2226571 (+) 774 WP_316729875.1 competence protein ComA -
  H2513_RS10490 (H2513_10470) - 2226611..2227126 (+) 516 WP_005751904.1 hypothetical protein -
  H2513_RS10495 (H2513_10475) - 2227126..2227650 (+) 525 WP_005751903.1 hypothetical protein -
  H2513_RS10500 (H2513_10480) - 2227653..2228015 (+) 363 WP_005748555.1 pilus assembly protein PilP -
  H2513_RS10505 (H2513_10485) comE 2228025..2229359 (+) 1335 WP_203331791.1 type IV pilus secretin PilQ Machinery gene
  H2513_RS10510 (H2513_10490) aroK 2229542..2230069 (+) 528 WP_005717623.1 shikimate kinase AroK -
  H2513_RS10515 (H2513_10495) aroB 2230086..2231174 (+) 1089 WP_316729879.1 3-dehydroquinate synthase -
  H2513_RS10520 (H2513_10500) - 2231178..2232083 (+) 906 WP_316729881.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  H2513_RS10525 (H2513_10505) - 2232130..2232435 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  H2513_RS10530 (H2513_10510) secM 2232528..2232839 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49256.68 Da        Isoelectric Point: 8.0309

>NTDB_id=471484 H2513_RS10505 WP_203331791.1 2228025..2229359(+) (comE) [Pasteurella multocida strain RCAD0730]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNS
TPVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVIAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=471484 H2513_RS10505 WP_203331791.1 2228025..2229359(+) (comE) [Pasteurella multocida strain RCAD0730]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTTGAGTTGCATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCTAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGACAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAGT
ACACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCATCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.5

97.297

0.608
  comE Haemophilus influenzae 86-028NP

61.806

97.297

0.601
  comE Glaesserella parasuis strain SC1401

49.192

97.523

0.48
  pilQ Vibrio campbellii strain DS40M4

39.524

94.595

0.374
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367