Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   H3M14_RS09325 Genome accession   NZ_CP059697
Coordinates   1826121..1827458 (-) Length   445 a.a.
NCBI ID   WP_052039239.1    Uniprot ID   A0AAE8J6D0
Organism   Latilactobacillus sakei strain CBA3635     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1821121..1832458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3M14_RS09310 (H3M14_09310) secA 1822166..1824529 (-) 2364 WP_011374203.1 preprotein translocase subunit SecA -
  H3M14_RS09315 (H3M14_09315) hpf 1824778..1825323 (-) 546 WP_011374202.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  H3M14_RS09320 (H3M14_09320) comFC 1825450..1825878 (-) 429 WP_154255254.1 ComF family protein Machinery gene
  H3M14_RS09325 (H3M14_09325) comFA 1826121..1827458 (-) 1338 WP_052039239.1 DEAD/DEAH box helicase Machinery gene
  H3M14_RS09330 (H3M14_09330) - 1827520..1828185 (+) 666 WP_025016329.1 YigZ family protein -
  H3M14_RS09335 (H3M14_09335) - 1828212..1829300 (-) 1089 WP_035144637.1 glycosyltransferase family 4 protein -
  H3M14_RS09340 (H3M14_09340) rny 1829456..1831021 (-) 1566 WP_011374197.1 ribonuclease Y -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 50353.78 Da        Isoelectric Point: 9.7862

>NTDB_id=471414 H3M14_RS09325 WP_052039239.1 1826121..1827458(-) (comFA) [Latilactobacillus sakei strain CBA3635]
MGQQVIACGRQFTAAQLADTQNNNYSLPQIKRRPAFLRVKHRLVCQRCQQVVPPQTCLPDGRHYCAQCLLFGRLVEGDWL
YTVPECHLFENNIPKLTWEGQLTPHQEQAAQAVVEVVQTQKRHVLTAVTGAGKTEMLFQGILVALQKGQRVCLAAPRVAV
CLELYPRLQAAFATASIMLMHGEQTEPYRYTQLVICTTHQLLKFYHAFDTVIVDEVDAFPFVDNPVLVTAVEQACKPQCA
LLYLTATPTPAIKRAIAAKQMTVSELPLRFHGHLLPEPHRHVAFNWRAQLKKGRLPKRLERDCQTCLKTQQILLFVPQVR
LLQPVANRLSTLLPAVRIETLHANDPDQIKKITAFRAQQIQLMVTTTILERGVTFKNVAVLVLGAEHMVFNEAVLVQIAG
RAGRHKDYAQNPVHFYYQDYTRAIKGACRQIKAQNQRGRRLQSTV

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=471414 H3M14_RS09325 WP_052039239.1 1826121..1827458(-) (comFA) [Latilactobacillus sakei strain CBA3635]
ATGGGACAACAAGTAATTGCCTGTGGTCGCCAGTTTACGGCGGCCCAGTTAGCCGATACGCAAAATAATAACTATAGTTT
ACCACAGATTAAGCGGCGACCTGCGTTTTTACGGGTCAAGCATCGCCTAGTCTGCCAACGTTGTCAGCAAGTGGTGCCGC
CGCAAACCTGCTTACCAGATGGGCGCCATTATTGCGCGCAATGTTTGTTATTTGGTCGTTTGGTAGAGGGCGATTGGTTA
TACACGGTACCGGAGTGTCATTTATTCGAAAATAACATACCAAAGTTGACGTGGGAGGGGCAATTAACACCGCATCAGGA
ACAAGCTGCCCAAGCGGTGGTTGAGGTGGTTCAAACACAAAAACGGCACGTATTAACGGCCGTGACCGGCGCAGGGAAGA
CCGAGATGCTTTTTCAAGGAATTTTAGTTGCGCTTCAAAAGGGTCAGCGGGTATGTCTGGCAGCGCCTAGAGTCGCCGTT
TGCTTGGAACTTTATCCGCGCCTGCAAGCTGCCTTTGCAACGGCTAGTATCATGTTGATGCACGGTGAGCAAACTGAGCC
CTATCGCTATACGCAGTTAGTGATTTGCACGACGCATCAGTTGCTGAAGTTTTATCACGCCTTTGACACTGTGATTGTCG
ATGAAGTCGATGCTTTTCCTTTTGTTGATAATCCCGTTTTAGTCACGGCAGTTGAACAAGCCTGCAAGCCCCAATGTGCT
TTATTATATTTAACGGCGACTCCGACCCCCGCTATTAAACGGGCAATTGCTGCTAAACAAATGACGGTTAGCGAATTACC
GCTCCGCTTTCATGGTCATCTATTACCAGAACCACATAGGCACGTCGCTTTTAATTGGCGGGCCCAATTAAAAAAAGGTC
GGTTACCTAAACGACTTGAGCGGGATTGCCAGACTTGTTTAAAAACACAACAGATTTTGTTATTCGTGCCGCAAGTGCGG
TTATTGCAACCAGTTGCCAATCGGTTATCAACCTTATTGCCGGCGGTCAGGATTGAAACATTGCATGCTAACGATCCGGA
TCAGATTAAAAAGATTACGGCGTTTAGAGCACAACAAATTCAGCTAATGGTGACGACCACCATTTTAGAACGGGGCGTTA
CGTTTAAAAACGTTGCAGTTTTGGTCTTAGGCGCGGAACACATGGTGTTTAACGAAGCCGTTTTGGTCCAGATTGCCGGG
CGAGCGGGACGGCATAAAGATTATGCCCAAAATCCGGTTCATTTTTATTATCAAGACTATACGCGGGCGATCAAAGGCGC
TTGTCGGCAAATTAAGGCCCAAAATCAGAGGGGACGGCGATTACAATCAACTGTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(307-405)

Type III restriction enzyme, res subunit (ResIII)

(101-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

98.652

100

0.987
  comFA/cflA Streptococcus pneumoniae TIGR4

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae D39

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae R6

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae Rx1

38.747

96.854

0.375
  comFA/cflA Streptococcus mitis SK321

38.515

96.854

0.373
  comFA/cflA Streptococcus mitis NCTC 12261

38.283

96.854

0.371
  comFA Bacillus subtilis subsp. subtilis str. 168

39.268

92.135

0.362