Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H3L94_RS06585 Genome accession   NZ_CP059567
Coordinates   1354193..1356322 (+) Length   709 a.a.
NCBI ID   WP_182121366.1    Uniprot ID   A0A7D7S688
Organism   Neisseria shayeganii strain DSM 22244     
Function   pseudopilus extrusion and retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1349193..1361322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L94_RS06565 (H3L94_06565) pilM 1351347..1352378 (+) 1032 WP_244958722.1 type IV pilus assembly protein PilM -
  H3L94_RS06570 (H3L94_06570) - 1352381..1352965 (+) 585 WP_182121363.1 PilN domain-containing protein -
  H3L94_RS06575 (H3L94_06575) pilO 1352987..1353601 (+) 615 WP_182121364.1 type 4a pilus biogenesis protein PilO -
  H3L94_RS06580 (H3L94_06580) - 1353631..1354173 (+) 543 WP_182121365.1 pilus assembly protein PilP -
  H3L94_RS06585 (H3L94_06585) pilQ 1354193..1356322 (+) 2130 WP_182121366.1 type IV pilus secretin PilQ Machinery gene
  H3L94_RS06590 (H3L94_06590) - 1356360..1356908 (+) 549 WP_182121367.1 shikimate kinase -
  H3L94_RS06595 (H3L94_06595) aroB 1356908..1357984 (+) 1077 WP_182123124.1 3-dehydroquinate synthase -
  H3L94_RS06600 (H3L94_06600) recG 1358163..1360199 (+) 2037 WP_182121368.1 ATP-dependent DNA helicase RecG Machinery gene
  H3L94_RS06605 (H3L94_06605) - 1360238..1360708 (-) 471 WP_182121369.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  H3L94_RS06610 (H3L94_06610) - 1360732..1361241 (-) 510 WP_182121370.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 709 a.a.        Molecular weight: 77456.59 Da        Isoelectric Point: 9.5523

>NTDB_id=470778 H3L94_RS06585 WP_182121366.1 1354193..1356322(+) (pilQ) [Neisseria shayeganii strain DSM 22244]
MKIKKLKYLSTLGLGLAIQSVFAGNITDINVSALPNNQKVIKIRFDRDMIKPNGFITTAPSRIALDFAGTGSNLNQPVLQ
YNDDLLSQIAVAQDNTKTRVLLSLNKPGQYNAEIKGNEVWVYINESTHTTAQTPTRHQTAAPALRQETVTITESASKANS
SASSESSNIANINVDFRKGANNTGIVEINPAGYRGNPDIKQHNDRLVILFKNNQIPIAAQRNFDVTDFSTPVRTVSLRRL
GNDTEITIRTQGNWEYKTSQSAGRYMLTISPKTVVAESGSDRRQRNFNGKRISLDFQDVEVRTILQILAKESGMNIVASD
SVSGKMTLSLKEVPWDQALDLVMQARDLDYRRNGNIINVAPRQELVDKERAVLEGRKAIDELGALHSRTFQLRYKDVNEF
RKILNISDAGGSSNSNDRNSLLSSRGSAVIDPATNTLIIKDNANVIKQFEDLIAQLDVPARQVMIEARIVEADDGFSRAL
GVRFGFGSENGGTKIGGRASGSLGDFGSSRWGIPNVNLPISSPTSAISVIKSYATGALGLELQAMQEQNRGKIISSPRVL
TQDRKQATIKSGTEIPYQEATSSGATSVSFKEAVLSMIVTPQITPDGNVIMDIQLNKDSVDRNCSVQGTPCISTKELNTK
AMVEDGGTIILGGIYEEENSTGEYKVPLLGDIPVIGNLFKSTTRSNTKRELLIFLTPRIMGGEGNVLRY

Nucleotide


Download         Length: 2130 bp        

>NTDB_id=470778 H3L94_RS06585 WP_182121366.1 1354193..1356322(+) (pilQ) [Neisseria shayeganii strain DSM 22244]
ATGAAAATTAAAAAGTTGAAATATCTAAGCACTTTAGGGTTGGGCTTGGCAATTCAAAGCGTTTTTGCCGGAAATATTAC
AGATATTAATGTATCCGCCTTACCCAATAACCAAAAAGTCATCAAAATTCGTTTTGATCGCGATATGATCAAACCGAATG
GCTTCATCACTACCGCTCCTTCGCGCATTGCATTAGATTTCGCAGGCACGGGATCCAATCTCAATCAACCTGTTTTGCAA
TACAATGACGATTTATTAAGTCAAATTGCTGTTGCCCAAGACAACACTAAAACCCGTGTCTTACTGAGTCTGAACAAACC
AGGCCAATACAATGCAGAAATAAAAGGCAATGAAGTATGGGTATACATTAACGAATCCACGCATACTACTGCCCAAACAC
CCACCAGACATCAAACAGCAGCCCCTGCTTTGCGTCAAGAAACAGTAACCATCACTGAATCGGCCTCAAAAGCAAACTCT
TCTGCTTCCTCAGAAAGCAGCAACATCGCCAATATCAATGTAGATTTTAGAAAGGGTGCCAATAATACGGGGATTGTTGA
AATCAATCCGGCTGGATACCGTGGCAATCCCGACATTAAACAACACAACGATCGCTTGGTTATTCTTTTCAAAAACAACC
AAATCCCTATTGCTGCCCAGCGAAATTTTGATGTAACGGATTTCAGTACTCCTGTCCGCACGGTAAGTTTACGCCGCCTG
GGTAACGATACGGAAATCACCATCCGTACACAGGGCAACTGGGAATACAAAACCAGCCAAAGTGCGGGACGCTATATGCT
GACCATCTCACCCAAAACCGTTGTAGCCGAGTCCGGCAGCGATCGCCGTCAGCGAAACTTCAATGGAAAACGTATTTCTC
TAGATTTTCAAGATGTTGAGGTGCGAACCATCCTGCAAATTTTGGCCAAAGAATCTGGTATGAATATCGTTGCCAGCGAC
AGCGTTTCTGGCAAAATGACTCTATCTTTGAAAGAAGTACCCTGGGATCAGGCTTTAGATTTGGTCATGCAAGCGCGAGA
CTTAGACTACCGTCGCAATGGTAATATTATCAATGTTGCGCCACGACAAGAATTGGTTGATAAAGAAAGAGCCGTTCTAG
AGGGACGCAAAGCAATTGACGAACTGGGAGCACTCCACTCCCGAACCTTCCAATTACGCTACAAAGATGTCAACGAATTC
CGCAAAATTTTGAACATCAGCGACGCCGGAGGATCGTCGAACTCGAATGACCGTAATTCTCTTTTAAGCAGCCGGGGCAG
CGCTGTTATCGATCCGGCAACCAACACGCTCATCATCAAGGACAATGCCAATGTAATCAAACAGTTTGAAGATTTGATTG
CCCAATTGGACGTGCCCGCCCGCCAAGTGATGATTGAAGCACGTATTGTGGAAGCTGATGACGGCTTTTCCCGCGCACTG
GGCGTGCGTTTCGGATTCGGTAGCGAAAACGGAGGCACCAAAATAGGAGGACGTGCTTCAGGATCACTGGGAGATTTCGG
TAGCAGCCGCTGGGGCATTCCCAATGTCAATTTGCCCATTAGCAGTCCGACTTCCGCTATTTCTGTCATCAAAAGTTATG
CAACAGGCGCTTTGGGTTTAGAACTCCAAGCAATGCAGGAACAAAATCGCGGCAAAATTATTTCCAGCCCTCGTGTGCTG
ACCCAAGACCGCAAACAGGCTACGATCAAATCAGGTACGGAAATTCCGTATCAAGAAGCCACTTCTAGCGGAGCCACCAG
CGTGAGCTTTAAAGAAGCCGTATTGTCCATGATTGTGACACCGCAAATCACACCTGATGGCAATGTAATCATGGACATCC
AGCTGAATAAAGATTCGGTTGACAGGAACTGTAGTGTACAAGGCACCCCCTGTATCAGCACCAAAGAGCTGAATACCAAA
GCTATGGTAGAAGACGGAGGCACCATCATCTTAGGTGGTATTTACGAAGAAGAAAATTCAACCGGCGAGTACAAAGTGCC
TTTGTTGGGTGACATTCCGGTAATCGGCAACCTGTTCAAAAGCACAACCCGTTCCAATACCAAACGCGAGTTGCTGATTT
TCTTGACCCCCCGCATTATGGGTGGGGAAGGCAATGTTTTGCGTTATTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(388-462)

Secretin and TonB N terminus short domain (STN)

(315-359)

Bacterial type II and III secretion system protein (Secretin)

(544-700)

AMIN domain (AMIN)

(37-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D7S688

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria meningitidis MC58

48.645

100

0.532
  pilQ Neisseria meningitidis M1080

48.892

100

0.529
  pilQ Neisseria gonorrhoeae strain FA1090

51.374

100

0.528