Detailed information NTG0470542

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H2788_RS14000
• Genome accession: NZ_CP059550
• Coordinates: 2990744..2991862 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain E-022166
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2985744..2996862

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H2788_RS13985 (H2788_13895)	-	2987911..2988753 (-)	843	WP_200027693.1	undecaprenyl-diphosphate phosphatase	-
H2788_RS13990 (H2788_13900)	-	2988860..2989552 (-)	693	WP_347016408.1	YggS family pyridoxal phosphate-dependent enzyme	-
H2788_RS13995 (H2788_13905)	pilT	2989679..2990716 (+)	1038	WP_019456823.1	type IV pilus twitching motility protein PilT	Machinery gene
H2788_RS14000 (H2788_13910)	pilU	2990744..2991862 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H2788_RS14005 (H2788_13915)	fur	2991932..2992369 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
H2788_RS14010 (H2788_13920)	bamE	2992481..2992879 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
H2788_RS14015 (H2788_13925)	-	2992935..2993258 (-)	324	WP_347016409.1	RnfH family protein	-
H2788_RS14020 (H2788_13930)	-	2993258..2994325 (-)	1068	WP_347016410.1	hypothetical protein	-
H2788_RS14025 (H2788_13935)	-	2994478..2994933 (-)	456	WP_004703295.1	bacteriohemerythrin	-
H2788_RS14030 (H2788_13940)	-	2995184..2996032 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=470542 H2788_RS14000 WP_004703285.1 2990744..2991862(+) (pilU) [Acinetobacter seifertii strain E-022166]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=470542 H2788_RS14000 WP_004703285.1 2990744..2991862(+) (pilU) [Acinetobacter seifertii strain E-022166]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCACCATC
GATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGCGAAGTGATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCGGGCATGGTTTTACGTCGAATTGAAACGAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGTGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GATCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCATTCGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCTGATGTCATCTTAATTGGTGAGATCCGTTCCCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTTAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAA
GCTTTATTTGACTTATATAAAACAGGTCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGCCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGATCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401