Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   H2N97_RS20510 Genome accession   NZ_CP059497
Coordinates   4119704..4120222 (-) Length   172 a.a.
NCBI ID   WP_004392979.1    Uniprot ID   A0A9Q3LJE1
Organism   Bacillus velezensis strain JSRB 08     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4109314..4120549 4119704..4120222 within 0


Gene organization within MGE regions


Location: 4109314..4120549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2N97_RS20445 (H2N97_20445) - 4109314..4109470 (+) 157 Protein_4016 DUF255 domain-containing protein -
  H2N97_RS20450 (H2N97_20450) - 4109632..4110444 (+) 813 WP_048367898.1 MerR family transcriptional regulator -
  H2N97_RS20455 (H2N97_20455) - 4110513..4111202 (+) 690 WP_110085264.1 DUF554 domain-containing protein -
  H2N97_RS20460 (H2N97_20460) - 4111349..4111504 (+) 156 Protein_4019 DUF255 domain-containing protein -
  H2N97_RS20465 (H2N97_20465) - 4111540..4113429 (+) 1890 WP_134986804.1 thioredoxin domain-containing protein -
  H2N97_RS20470 (H2N97_20470) - 4113426..4114322 (-) 897 WP_017418443.1 CPBP family intramembrane glutamic endopeptidase -
  H2N97_RS20475 (H2N97_20475) - 4114537..4115868 (+) 1332 WP_014419380.1 MFS transporter -
  H2N97_RS20480 (H2N97_20480) - 4115902..4116282 (-) 381 WP_017418442.1 VOC family protein -
  H2N97_RS20485 (H2N97_20485) - 4116339..4117277 (-) 939 WP_017418441.1 LacI family DNA-binding transcriptional regulator -
  H2N97_RS20490 (H2N97_20490) xth 4117340..4118098 (-) 759 WP_017418440.1 exodeoxyribonuclease III -
  H2N97_RS20495 (H2N97_20495) - 4118095..4118652 (-) 558 WP_017418439.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  H2N97_RS20500 (H2N97_20500) - 4118649..4119230 (-) 582 WP_017418438.1 bifunctional transcriptional activator/DNA repair enzyme AdaA -
  H2N97_RS20505 (H2N97_20505) rpsR 4119421..4119660 (-) 240 WP_004392983.1 30S ribosomal protein S18 -
  H2N97_RS20510 (H2N97_20510) ssbA 4119704..4120222 (-) 519 WP_004392979.1 single-stranded DNA-binding protein SsbA Machinery gene
  H2N97_RS20515 (H2N97_20515) rpsF 4120262..4120549 (-) 288 WP_004392977.1 30S ribosomal protein S6 -

Sequence


Protein


Download         Length: 172 a.a.        Molecular weight: 18745.31 Da        Isoelectric Point: 4.7621

>NTDB_id=470249 H2N97_RS20510 WP_004392979.1 4119704..4120222(-) (ssbA) [Bacillus velezensis strain JSRB 08]
MLNRVVLVGRLTKDPELRYTPSGAAVATFTLAVNRTFTNQSGEREADFINCVTWRRQAENVANFLKKGSLAGVDGRLQTR
NYENQQGQRVFVTEVQAESVQFLEPKNSGGSGSGGYNEGNSGGGQYFGGGQNDNPFGGNQNNQRRNQGNSFNDDPFANDG
KPIDISDDDLPF

Nucleotide


Download         Length: 519 bp        

>NTDB_id=470249 H2N97_RS20510 WP_004392979.1 4119704..4120222(-) (ssbA) [Bacillus velezensis strain JSRB 08]
ATGCTTAACCGAGTTGTATTAGTCGGAAGACTGACAAAAGACCCAGAGCTTCGTTATACGCCAAGCGGTGCGGCTGTCGC
CACGTTTACTCTTGCTGTGAATCGTACATTCACGAACCAGTCCGGAGAACGTGAAGCCGATTTCATTAATTGTGTCACTT
GGAGAAGACAAGCCGAAAACGTTGCAAACTTTCTTAAAAAAGGAAGCCTTGCCGGCGTAGACGGCCGACTCCAGACAAGA
AATTATGAAAACCAGCAGGGACAGCGTGTCTTCGTGACAGAAGTCCAAGCTGAAAGTGTTCAATTTCTTGAGCCTAAAAA
CAGCGGCGGTTCTGGATCAGGCGGATACAACGAAGGAAACAGCGGCGGAGGCCAATACTTTGGCGGAGGCCAAAATGATA
ATCCGTTCGGCGGAAATCAGAACAACCAGAGACGAAATCAAGGGAACAGCTTTAATGATGATCCATTTGCCAACGACGGC
AAGCCGATTGACATCTCGGATGATGATCTTCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

98.837

100

0.988

  ssb Latilactobacillus sakei subsp. sakei 23K

58.757

100

0.605

  ssbB Bacillus subtilis subsp. subtilis str. 168

64.151

61.628

0.395

  ssb Glaesserella parasuis strain SC1401

35.519

100

0.378