Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   JSQ74_RS10570 Genome accession   NZ_CP069710
Coordinates   2193786..2195279 (-) Length   497 a.a.
NCBI ID   WP_000249356.1    Uniprot ID   -
Organism   Escherichia coli strain EA2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2188786..2200279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ74_RS10540 (JSQ74_10540) gspK 2189580..2190557 (-) 978 WP_204084398.1 type II secretion system minor pseudopilin GspK -
  JSQ74_RS10545 (JSQ74_10545) gspJ 2190560..2191159 (-) 600 WP_096998419.1 type II secretion system minor pseudopilin GspJ -
  JSQ74_RS10550 (JSQ74_10550) gspI 2191156..2191527 (-) 372 WP_000820111.1 type II secretion system minor pseudopilin GspI -
  JSQ74_RS10555 (JSQ74_10555) gspH 2191524..2192087 (-) 564 WP_047084647.1 type II secretion system minor pseudopilin GspH -
  JSQ74_RS10560 (JSQ74_10560) gspG 2192091..2192546 (-) 456 WP_000287130.1 type II secretion system major pseudopilin GspG -
  JSQ74_RS10565 (JSQ74_10565) gspF 2192563..2193786 (-) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  JSQ74_RS10570 (JSQ74_10570) pilB/pilB1 2193786..2195279 (-) 1494 WP_000249356.1 type II secretion system ATPase GspE Machinery gene
  JSQ74_RS10575 (JSQ74_10575) gspD 2195279..2197339 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  JSQ74_RS10580 (JSQ74_10580) gspC 2197369..2198328 (-) 960 WP_096998418.1 type II secretion system protein GspC -
  JSQ74_RS10585 (JSQ74_10585) gspS2 2198346..2198756 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  JSQ74_RS10590 (JSQ74_10590) pilD 2198822..2199631 (-) 810 WP_096998417.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55232.41 Da        Isoelectric Point: 6.0390

>NTDB_id=470013 JSQ74_RS10570 WP_000249356.1 2193786..2195279(-) (pilB/pilB1) [Escherichia coli strain EA2]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=470013 JSQ74_RS10570 WP_000249356.1 2193786..2195279(-) (pilB/pilB1) [Escherichia coli strain EA2]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCAGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGAATCAAGGTAATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGACGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACA
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362