Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H2515_RS14400 Genome accession   NZ_CP059488
Coordinates   2879854..2881563 (-) Length   569 a.a.
NCBI ID   WP_198660458.1    Uniprot ID   A0A7T4WDR5
Organism   Acidithiobacillus ferrivorans strain XJFY6S-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2874854..2886563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H2515_RS14380 (H2515_14295) coaE 2875375..2875977 (-) 603 WP_035190775.1 dephospho-CoA kinase -
  H2515_RS14385 (H2515_14300) - 2875978..2876778 (-) 801 WP_035190771.1 A24 family peptidase -
  H2515_RS14390 (H2515_14305) pilR 2876775..2878130 (-) 1356 WP_269802048.1 sigma-54 dependent transcriptional regulator Regulator
  H2515_RS14395 (H2515_14310) - 2878127..2879728 (-) 1602 WP_176712639.1 nitrogen regulation protein NR(II) -
  H2515_RS14400 (H2515_14315) pilB 2879854..2881563 (-) 1710 WP_198660458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H2515_RS14405 (H2515_14320) aroE 2881533..2882432 (-) 900 WP_198660459.1 shikimate dehydrogenase -
  H2515_RS14410 (H2515_14325) - 2882410..2883573 (-) 1164 WP_232821422.1 anhydro-N-acetylmuramic acid kinase -
  H2515_RS14415 (H2515_14330) - 2883641..2884129 (-) 489 WP_035190764.1 peptidylprolyl isomerase -
  H2515_RS14420 (H2515_14335) hemH 2884192..2885205 (-) 1014 WP_198660461.1 ferrochelatase -
  H2515_RS14425 (H2515_14340) xth 2885202..2885975 (-) 774 WP_035190758.1 exodeoxyribonuclease III -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62625.46 Da        Isoelectric Point: 6.3584

>NTDB_id=470008 H2515_RS14400 WP_198660458.1 2879854..2881563(-) (pilB) [Acidithiobacillus ferrivorans strain XJFY6S-08]
MATNPAHIALTPVLRALVSNGLSDEAQLQSLAADPARGKMPLLFYAVEKGAVPATVLMAHLSARYNMPMLDLDAVMINEL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNTGLQVLPILVEANKLVKAVNAATNSMQGGIDAVFMD
KPRDEEEQEEFDLAQDNDGNVEDAPVVRFARQLLLDAIQRGVSDIHLEPYEKDFRVRYRLDGVLQDVLHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSKVPIEQLGFLPDQRKAFEVAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLSFAAALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGGVHLVMAQRLARKLCPHCKKPLRIPESVLIEAGF
APEDLQGWRPLGAAGCDQCNNTGYKGRMGLYQVMPVSDAMREIIMRGGTSIDLARQAAQEGVLTMRQNGLRRIKEGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=470008 H2515_RS14400 WP_198660458.1 2879854..2881563(-) (pilB) [Acidithiobacillus ferrivorans strain XJFY6S-08]
ATGGCTACAAATCCAGCACACATTGCGTTGACGCCCGTATTGCGGGCATTAGTCAGCAACGGGCTAAGCGACGAGGCACA
GTTGCAAAGCCTTGCCGCCGATCCGGCGCGTGGCAAGATGCCGCTGCTGTTTTACGCGGTTGAGAAGGGGGCAGTTCCTG
CCACTGTGCTGATGGCGCACCTTTCGGCGCGCTATAACATGCCCATGCTCGATCTGGATGCAGTCATGATTAATGAGTTG
CTCATCCGGAAGCTCGACAAAGGACTGATGACCCGCTATCTGGTGCTGCCCTTGTCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCAGATCCTACGGATTTTAAGGCGGTGGAAGATGTGAAGTTCAACACCGGCCTGCAGGTATTGCCGATACTGG
TCGAGGCCAATAAGTTGGTAAAGGCGGTGAATGCCGCGACCAATAGTATGCAAGGCGGGATTGACGCTGTTTTCATGGAT
AAACCGCGCGACGAAGAAGAGCAGGAAGAATTCGATCTTGCCCAGGACAATGATGGCAATGTCGAGGATGCGCCCGTGGT
GCGTTTTGCCCGGCAATTGCTGCTGGATGCCATTCAACGCGGGGTTTCCGATATTCATCTGGAACCCTATGAAAAGGATT
TTCGGGTGCGTTACCGCCTCGATGGCGTATTGCAGGATGTGCTGCATCCGCCGGTGGCGTTGCGTGATGGTGTCACTTCG
CGCCTGAAGATTCTCTGTCGCCTGGATATTTCTGAGCGGCGTTTACCCCAGGATGGCCGTCTGCGGGTCAGGGTGCCACC
GGCACGGGTTATTGATTTCCGGGTCTCTTTTTTGCCCACCAGTTTTGGCGAAACCATTGTGCTGCGCTTGCTGGATCCGG
CCAGTTCCAAGGTGCCCATAGAACAATTGGGCTTTTTGCCAGACCAGCGCAAAGCTTTTGAAGTGGCTATCCACCGGCCC
TACGGTATGATCCTGGTCACCGGCCCCACGGGTTCGGGTAAAACAACGACCCTCTACACCGCGCTGAATATTCTCAACAC
CGGCGACTGCAACATCAGTACCGCAGAGGATCCTGTGGAAATCCCGGTATACGGGATCAATCAGGTGAATATCAACGAGC
GTATCGGTCTCAGCTTTGCCGCGGCCTTGCGTTCCTTCCTGCGCCAGGATCCTGACATCATTATGGTCGGTGAGGTGCGT
GATCTGGAAACCGCGGAAACGGCCATCAAAGCCGCCCAGACGGGTCATCTGGTGTTGGCCACCCTGCATACCAATGATGC
ACCACAGAGTCTCACCCGTCTGGAAAATATGGGCATCCCTACCTATAACATAGCGGGCGGTGTGCACCTGGTGATGGCGC
AACGACTCGCCCGCAAACTCTGCCCGCACTGTAAAAAGCCGCTGCGCATTCCCGAATCGGTCCTGATTGAGGCGGGATTT
GCGCCCGAGGATCTGCAGGGCTGGCGCCCTCTGGGCGCGGCGGGCTGTGATCAGTGCAATAACACCGGGTATAAAGGTCG
GATGGGACTCTATCAGGTGATGCCGGTGAGCGATGCTATGCGTGAAATCATTATGCGCGGAGGTACTTCCATCGACCTAG
CGCGGCAGGCGGCACAGGAGGGGGTACTGACCATGCGGCAAAATGGATTGAGGCGGATCAAGGAGGGCGTCACTAGCCTT
GAGGAGGTTTTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T4WDR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.606

100

0.49
  pilB Acinetobacter baumannii D1279779

46.329

100

0.466
  pilB Acinetobacter baylyi ADP1

45.884

100

0.46
  pilF Neisseria gonorrhoeae MS11

45.811

98.594

0.452
  pilB Vibrio cholerae strain A1552

47.249

92.619

0.438
  pilB Vibrio parahaemolyticus RIMD 2210633

43.106

100

0.434
  pilB Vibrio campbellii strain DS40M4

45.437

92.443

0.42
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.322

0.366