Detailed information NTG0469105

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H1I66_RS01525
• Genome accession: NZ_CP059358
• Coordinates: 330552..331610 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain 2018HLJAB1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 325552..336610

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1I66_RS01510 (H1I66_01505)	-	326657..326989 (+)	333	WP_000169594.1	hypothetical protein	-
H1I66_RS01515 (H1I66_01510)	-	326992..327816 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
H1I66_RS01520 (H1I66_01515)	ponA	327834..330389 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
H1I66_RS01525 (H1I66_01520)	pilM	330552..331610 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
H1I66_RS01530 (H1I66_01525)	pilN	331610..332251 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
H1I66_RS01535 (H1I66_01530)	pilO	332248..332988 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
H1I66_RS01540 (H1I66_01535)	pilP	332999..333526 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
H1I66_RS01545 (H1I66_01540)	pilQ	333594..335759 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
H1I66_RS01550 (H1I66_01545)	aroK	335771..336340 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=469105 H1I66_RS01525 WP_002014066.1 330552..331610(+) (pilM) [Acinetobacter baumannii strain 2018HLJAB1]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=469105 H1I66_RS01525 WP_002014066.1 330552..331610(+) (pilM) [Acinetobacter baumannii strain 2018HLJAB1]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423