Detailed information NTG0469037

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H1I71_RS14520
• Genome accession: NZ_CP059353
• Coordinates: 2982781..2983899 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain 2016LNAB1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2977781..2988899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1I71_RS14510 (H1I71_14415)	-	2980897..2981589 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
H1I71_RS14515 (H1I71_14420)	pilT	2981716..2982753 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H1I71_RS14520 (H1I71_14425)	pilU	2982781..2983899 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H1I71_RS14525 (H1I71_14430)	fur	2983969..2984406 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H1I71_RS14530 (H1I71_14435)	bamE	2984518..2984916 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H1I71_RS14535 (H1I71_14440)	-	2984971..2985294 (-)	324	WP_000442587.1	RnfH family protein	-
H1I71_RS14540 (H1I71_14445)	-	2985294..2986361 (-)	1068	WP_002019572.1	hypothetical protein	-
H1I71_RS14545 (H1I71_14450)	-	2986514..2986969 (-)	456	WP_000782976.1	bacteriohemerythrin	-
H1I71_RS14550 (H1I71_14455)	-	2987222..2988070 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=469037 H1I71_RS14520 WP_000347039.1 2982781..2983899(+) (pilU) [Acinetobacter baumannii strain 2016LNAB1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=469037 H1I71_RS14520 WP_000347039.1 2982781..2983899(+) (pilU) [Acinetobacter baumannii strain 2016LNAB1]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395