Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H1D00_RS02850 Genome accession   NZ_CP059330
Coordinates   530836..532149 (-) Length   437 a.a.
NCBI ID   WP_240021567.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0722     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525836..537149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D00_RS02820 (H1D00_02845) ruvB 526332..527339 (-) 1008 WP_240021565.1 Holliday junction branch migration DNA helicase RuvB -
  H1D00_RS02825 (H1D00_02850) ruvA 527476..528081 (-) 606 WP_006252204.1 Holliday junction branch migration protein RuvA -
  H1D00_RS02830 (H1D00_02855) - 528193..528816 (-) 624 WP_006247941.1 LysE family transporter -
  H1D00_RS02835 (H1D00_02860) - 528823..529296 (-) 474 WP_240018882.1 phosphatidylglycerophosphatase A -
  H1D00_RS02840 (H1D00_02865) thiL 529301..530287 (-) 987 WP_240021822.1 thiamine-phosphate kinase -
  H1D00_RS02845 (H1D00_02870) nusB 530343..530756 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  H1D00_RS02850 (H1D00_02875) comE 530836..532149 (-) 1314 WP_240021567.1 type IV pilus secretin PilQ Machinery gene
  H1D00_RS02855 (H1D00_02880) - 532158..532571 (-) 414 WP_006247936.1 hypothetical protein -
  H1D00_RS02860 (H1D00_02885) - 532574..533110 (-) 537 WP_006247935.1 hypothetical protein -
  H1D00_RS02865 (H1D00_02890) - 533267..533632 (-) 366 WP_240021569.1 hypothetical protein -
  H1D00_RS02870 (H1D00_02895) - 533608..534306 (-) 699 WP_240017830.1 competence protein ComA -
  H1D00_RS02875 (H1D00_02900) - 534430..536997 (+) 2568 WP_240021570.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48449.90 Da        Isoelectric Point: 6.9846

>NTDB_id=468742 H1D00_RS02850 WP_240021567.1 530836..532149(-) (comE) [Mannheimia haemolytica strain 0722]
MWRIFIYFLCGFSLAYANTISIVLXNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKXEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=468742 H1D00_RS02850 WP_240021567.1 530836..532149(-) (comE) [Mannheimia haemolytica strain 0722]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTANAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAANAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCACAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.073

99.771

0.629
  comE Haemophilus influenzae Rd KW20

51.034

99.542

0.508
  comE Haemophilus influenzae 86-028NP

50.345

99.542

0.501
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.955

96.339

0.375
  pilQ Vibrio cholerae strain A1552

38.955

96.339

0.375
  pilQ Vibrio campbellii strain DS40M4

37.123

98.627

0.366