Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H1D01_RS10445 Genome accession   NZ_CP059329
Coordinates   2058584..2059909 (-) Length   441 a.a.
NCBI ID   WP_240025195.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0727     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2053584..2064909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D01_RS10415 (H1D01_10435) ruvB 2054095..2055099 (-) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -
  H1D01_RS10420 (H1D01_10440) ruvA 2055246..2055839 (-) 594 Protein_2021 Holliday junction branch migration protein RuvA -
  H1D01_RS10425 (H1D01_10445) - 2055951..2056574 (-) 624 WP_006247941.1 LysE family transporter -
  H1D01_RS10430 - 2056581..2057054 (-) 474 WP_240018882.1 phosphatidylglycerophosphatase A -
  H1D01_RS10435 (H1D01_10450) thiL 2057064..2058038 (-) 975 Protein_2024 thiamine-phosphate kinase -
  H1D01_RS10440 (H1D01_10455) nusB 2058101..2058514 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  H1D01_RS10445 (H1D01_10460) comE 2058584..2059909 (-) 1326 WP_240025195.1 type IV pilus secretin PilQ Machinery gene
  H1D01_RS10450 (H1D01_10465) - 2059918..2060331 (-) 414 WP_240025197.1 hypothetical protein -
  H1D01_RS10455 (H1D01_10470) - 2060334..2060870 (-) 537 WP_006247935.1 hypothetical protein -
  H1D01_RS10460 (H1D01_10475) - 2060872..2061393 (-) 522 WP_006247934.1 hypothetical protein -
  H1D01_RS10465 (H1D01_10480) - 2061369..2062067 (-) 699 WP_240017830.1 competence protein ComA -
  H1D01_RS10470 (H1D01_10485) - 2062191..2064758 (+) 2568 WP_240025199.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48891.02 Da        Isoelectric Point: 6.9251

>NTDB_id=468731 H1D01_RS10445 WP_240025195.1 2058584..2059909(-) (comE) [Mannheimia haemolytica strain 0727]
MWRIFIYFLCGFSLAYANTISIVLXNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKXEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASXEVIESLTKGSGTFLSENGYIHFDERS
NSLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNV
NFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVL
GLEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFI
KRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKNQNSTS

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=468731 H1D01_RS10445 WP_240025195.1 2058584..2059909(-) (comE) [Mannheimia haemolytica strain 0727]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTANAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAANAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCCA
NNGAGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGC
AATAGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGA
GCAAATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTT
CTCGTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTA
AATTTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAG
TGCATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCA
AGCAAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTA
GGGCTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTC
CCAATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCA
TTGTACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATC
AAGCGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATC
TAATGAAAAGAGCAGCAACCGCAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(268-425)

Bacterial type II/III secretion system short domain (Secretin_N)

(58-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.785

97.052

0.619
  comE Haemophilus influenzae Rd KW20

50.337

100

0.508
  comE Haemophilus influenzae 86-028NP

50.229

98.866

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.915

95.692

0.363
  pilQ Vibrio cholerae strain A1552

37.915

95.692

0.363
  pilQ Vibrio campbellii strain DS40M4

37.209

97.506

0.363