Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H1D02_RS03025 Genome accession   NZ_CP059328
Coordinates   641619..642932 (+) Length   437 a.a.
NCBI ID   WP_240028073.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0728     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636619..647932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D02_RS03000 (H1D02_03030) - 636773..639337 (-) 2565 WP_240028072.1 penicillin-binding protein 1A -
  H1D02_RS03005 (H1D02_03035) - 639461..640159 (+) 699 WP_240020005.1 competence protein ComA -
  H1D02_RS03010 (H1D02_03040) - 640135..640656 (+) 522 WP_006247934.1 hypothetical protein -
  H1D02_RS03015 (H1D02_03045) - 640658..641194 (+) 537 WP_006247935.1 hypothetical protein -
  H1D02_RS03020 (H1D02_03050) - 641197..641610 (+) 414 WP_006247936.1 hypothetical protein -
  H1D02_RS03025 (H1D02_03055) comE 641619..642932 (+) 1314 WP_240028073.1 type IV pilus secretin PilQ Machinery gene
  H1D02_RS03030 (H1D02_03060) nusB 643012..643425 (+) 414 WP_006247938.1 transcription antitermination factor NusB -
  H1D02_RS03035 (H1D02_03065) thiL 643488..644468 (+) 981 WP_006247939.1 thiamine-phosphate kinase -
  H1D02_RS03040 (H1D02_03070) - 644473..644946 (+) 474 WP_240028074.1 phosphatidylglycerophosphatase A -
  H1D02_RS03045 (H1D02_03075) - 644953..645576 (+) 624 WP_240028075.1 LysE family transporter -
  H1D02_RS03050 (H1D02_03080) ruvA 645688..646293 (+) 606 WP_006252204.1 Holliday junction branch migration protein RuvA -
  H1D02_RS03055 (H1D02_03085) ruvB 646429..647433 (+) 1005 WP_006252203.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48464.91 Da        Isoelectric Point: 7.2360

>NTDB_id=468692 H1D02_RS03025 WP_240028073.1 641619..642932(+) (comE) [Mannheimia haemolytica strain 0728]
MWRIFIYFLCGFSLAYANTISIVXKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHXKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=468692 H1D02_RS03025 WP_240028073.1 641619..642932(+) (comE) [Mannheimia haemolytica strain 0728]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTNAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAANAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCACAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.073

99.771

0.629
  comE Haemophilus influenzae Rd KW20

50.805

99.542

0.506
  comE Haemophilus influenzae 86-028NP

50.115

99.542

0.499
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.192

96.339

0.378
  pilQ Vibrio cholerae strain A1552

39.192

96.339

0.378
  pilQ Vibrio campbellii strain DS40M4

37.355

98.627

0.368