Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H1D04_RS01055 Genome accession   NZ_CP059326
Coordinates   216337..217650 (+) Length   437 a.a.
NCBI ID   WP_275686612.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0745     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211337..222650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D04_RS01030 (H1D04_01010) - 211494..214058 (-) 2565 WP_275686611.1 penicillin-binding protein 1A -
  H1D04_RS01035 (H1D04_01015) - 214182..214880 (+) 699 WP_240020005.1 competence protein ComA -
  H1D04_RS01040 - 214856..215032 (+) 177 WP_240020006.1 hypothetical protein -
  H1D04_RS01045 (H1D04_01025) - 215376..215912 (+) 537 WP_006247935.1 hypothetical protein -
  H1D04_RS01050 (H1D04_01030) - 215915..216328 (+) 414 WP_006247936.1 hypothetical protein -
  H1D04_RS01055 (H1D04_01035) comE 216337..217650 (+) 1314 WP_275686612.1 type IV pilus secretin PilQ Machinery gene
  H1D04_RS01060 (H1D04_01040) nusB 217730..218143 (+) 414 WP_006247938.1 transcription antitermination factor NusB -
  H1D04_RS01065 (H1D04_01045) thiL 218199..219185 (+) 987 WP_275686901.1 thiamine-phosphate kinase -
  H1D04_RS01070 (H1D04_01050) - 219190..219663 (+) 474 WP_240028074.1 phosphatidylglycerophosphatase A -
  H1D04_RS01075 (H1D04_01055) - 219670..220293 (+) 624 WP_240029008.1 LysE family transporter -
  H1D04_RS01080 (H1D04_01060) ruvA 220405..221010 (+) 606 WP_006252204.1 Holliday junction branch migration protein RuvA -
  H1D04_RS01085 (H1D04_01065) ruvB 221146..222150 (+) 1005 WP_275686613.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48455.67 Da        Isoelectric Point: 7.2360

>NTDB_id=468644 H1D04_RS01055 WP_275686612.1 216337..217650(+) (comE) [Mannheimia haemolytica strain 0745]
MWRIFIYFLCGFSLAYANTISIVXKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHXKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTXKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=468644 H1D04_RS01055 WP_275686612.1 216337..217650(+) (comE) [Mannheimia haemolytica strain 0745]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTNAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAANAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCANAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCACAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.073

99.771

0.629
  comE Haemophilus influenzae Rd KW20

50.805

99.542

0.506
  comE Haemophilus influenzae 86-028NP

50.115

99.542

0.499
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.192

96.339

0.378
  pilQ Vibrio cholerae strain A1552

39.192

96.339

0.378
  pilQ Vibrio campbellii strain DS40M4

37.355

98.627

0.368