Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   H1D06_RS09725 Genome accession   NZ_CP059324
Coordinates   1924580..1925902 (-) Length   440 a.a.
NCBI ID   WP_240017828.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0760     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1919580..1930902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1D06_RS09695 (H1D06_09710) ruvB 1920090..1921094 (-) 1005 WP_006252203.1 Holliday junction branch migration DNA helicase RuvB -
  H1D06_RS09700 (H1D06_09715) ruvA 1921230..1921835 (-) 606 WP_006252204.1 Holliday junction branch migration protein RuvA -
  H1D06_RS09705 (H1D06_09720) - 1921947..1922570 (-) 624 WP_240017827.1 LysE family transporter -
  H1D06_RS09710 - 1922577..1923050 (-) 474 WP_240018882.1 phosphatidylglycerophosphatase A -
  H1D06_RS09715 (H1D06_09725) thiL 1923060..1924034 (-) 975 Protein_1866 thiamine-phosphate kinase -
  H1D06_RS09720 (H1D06_09730) nusB 1924097..1924510 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  H1D06_RS09725 (H1D06_09735) comE 1924580..1925902 (-) 1323 WP_240017828.1 type IV pilus secretin PilQ Machinery gene
  H1D06_RS09730 (H1D06_09740) - 1925911..1926324 (-) 414 WP_240017829.1 hypothetical protein -
  H1D06_RS09735 (H1D06_09745) - 1926327..1926863 (-) 537 WP_006247935.1 hypothetical protein -
  H1D06_RS09740 (H1D06_09750) - 1926865..1927386 (-) 522 WP_006247934.1 hypothetical protein -
  H1D06_RS09745 (H1D06_09755) - 1927362..1928060 (-) 699 WP_240017830.1 competence protein ComA -
  H1D06_RS09750 (H1D06_09760) - 1928184..1930748 (+) 2565 WP_240017831.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 48728.78 Da        Isoelectric Point: 6.4246

>NTDB_id=468617 H1D06_RS09725 WP_240017828.1 1924580..1925902(-) (comE) [Mannheimia haemolytica strain 0760]
MWRIFIYFLCGFSLAYANTISIVLXNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKXEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNDVTNNLNVN
FPITGGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQXGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNHKNQNSTS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=468617 H1D06_RS09725 WP_240017828.1 1924580..1925902(-) (comE) [Mannheimia haemolytica strain 0760]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTANAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAANAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACGATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTGCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAANAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCACAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)

Bacterial type II and III secretion system protein (Secretin)

(267-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.934

97.045

0.62
  comE Haemophilus influenzae Rd KW20

50.225

100

0.507
  comE Haemophilus influenzae 86-028NP

49.55

100

0.5
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.955

95.682

0.373
  pilQ Vibrio cholerae strain A1552

38.955

95.682

0.373
  pilQ Vibrio campbellii strain DS40M4

37.123

97.955

0.364