Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HS074_RS01140 Genome accession   NZ_CP059245
Coordinates   216976..218301 (+) Length   441 a.a.
NCBI ID   WP_409038563.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0740     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 211976..223301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS074_RS01115 (HS074_01100) - 212127..214691 (-) 2565 WP_409037971.1 penicillin-binding protein 1A -
  HS074_RS01120 (HS074_01105) - 214815..215513 (+) 699 WP_240020005.1 competence protein ComA -
  HS074_RS01125 (HS074_01110) - 215489..216010 (+) 522 WP_006247934.1 hypothetical protein -
  HS074_RS01130 (HS074_01115) - 216012..216551 (+) 540 WP_409038562.1 hypothetical protein -
  HS074_RS01135 (HS074_01120) - 216554..216967 (+) 414 WP_006247936.1 hypothetical protein -
  HS074_RS01140 (HS074_01125) comE 216976..218301 (+) 1326 WP_409038563.1 type IV pilus secretin PilQ Machinery gene
  HS074_RS01145 (HS074_01130) nusB 218371..218784 (+) 414 WP_006247938.1 transcription antitermination factor NusB -
  HS074_RS01150 (HS074_01135) thiL 218847..219827 (+) 981 WP_240029007.1 thiamine-phosphate kinase -
  HS074_RS01155 (HS074_01140) - 219832..220305 (+) 474 WP_240028074.1 phosphatidylglycerophosphatase A -
  HS074_RS01160 (HS074_01145) - 220312..220935 (+) 624 WP_409038564.1 LysE family transporter -
  HS074_RS01165 (HS074_01150) ruvA 221047..221652 (+) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  HS074_RS01170 (HS074_01155) ruvB 221789..222793 (+) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48900.31 Da        Isoelectric Point: 7.1883

>NTDB_id=468136 HS074_RS01140 WP_409038563.1 216976..218301(+) (comE) [Mannheimia haemolytica strain 0740]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHXKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASXEVIESLTKGSGTFLSENGYIHFDERS
NSLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNV
NFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVL
GLEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFI
KRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKNQNSTS

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=468136 HS074_RS01140 WP_409038563.1 216976..218301(+) (comE) [Mannheimia haemolytica strain 0740]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAANAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCCN
NAGAGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGC
AATAGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGA
GCAAATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTT
CTCGTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTA
AATTTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAG
TGCATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCA
AGCAAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTA
GGGCTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTC
CCAATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCA
TTGTACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATC
AAGCGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATC
TAATGAAAAGAGCAGCAACCGCAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(58-188)

Bacterial type II and III secretion system protein (Secretin)

(268-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.785

97.052

0.619
  comE Haemophilus influenzae Rd KW20

50.337

100

0.508
  comE Haemophilus influenzae 86-028NP

50.229

98.866

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.152

95.692

0.365
  pilQ Vibrio cholerae strain A1552

38.152

95.692

0.365
  pilQ Vibrio campbellii strain DS40M4

37.442

97.506

0.365