Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS054_RS12895 Genome accession   NZ_CP059244
Coordinates   2513529..2514008 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2508529..2519008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS054_RS12860 (HS054_12735) lptE 2508778..2509296 (+) 519 WP_409037689.1 LPS assembly lipoprotein LptE -
  HS054_RS12865 (HS054_12740) holA 2509339..2510364 (+) 1026 WP_409043435.1 DNA polymerase III subunit delta -
  HS054_RS12870 (HS054_12745) yacG 2510365..2510550 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS054_RS12875 (HS054_12750) coaE 2510532..2511167 (-) 636 WP_409043437.1 dephospho-CoA kinase -
  HS054_RS12880 (HS054_12755) - 2511330..2511860 (-) 531 Protein_2488 prepilin peptidase -
  HS054_RS12885 (HS054_12760) pilC 2511863..2513059 (-) 1197 WP_409042879.1 type II secretion system F family protein Machinery gene
  HS054_RS12890 (HS054_12765) - 2513065..2513508 (-) 444 Protein_2490 pilus assembly protein PilB -
  HS054_RS12895 (HS054_12770) pilA 2513529..2514008 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS054_RS12900 (HS054_12775) radA 2514188..2515573 (+) 1386 WP_409042880.1 DNA repair protein RadA -
  HS054_RS12905 (HS054_12780) - 2515628..2515858 (-) 231 WP_006248398.1 YgjV family protein -
  HS054_RS12910 (HS054_12785) - 2515977..2516471 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS054_RS12915 (HS054_12790) proB 2516583..2517668 (+) 1086 WP_240025850.1 glutamate 5-kinase -
  HS054_RS12920 (HS054_12795) - 2517668..2518432 (+) 765 WP_240018340.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=468134 HS054_RS12895 WP_006248401.1 2513529..2514008(-) (pilA) [Mannheimia haemolytica strain 0001]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468134 HS054_RS12895 WP_006248401.1 2513529..2514008(-) (pilA) [Mannheimia haemolytica strain 0001]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491