Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HS054_RS07335 Genome accession   NZ_CP059244
Coordinates   1453732..1455054 (-) Length   440 a.a.
NCBI ID   WP_409042642.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1448732..1460054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS054_RS07305 (HS054_07255) ruvB 1449241..1450245 (-) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -
  HS054_RS07310 (HS054_07260) ruvA 1450381..1450986 (-) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  HS054_RS07315 (HS054_07265) - 1451098..1451721 (-) 624 WP_006247941.1 LysE family transporter -
  HS054_RS07320 (HS054_07270) - 1451728..1452201 (-) 474 WP_006247940.1 phosphatidylglycerophosphatase A -
  HS054_RS07325 (HS054_07275) thiL 1452206..1453186 (-) 981 WP_006247939.1 thiamine-phosphate kinase -
  HS054_RS07330 (HS054_07280) nusB 1453249..1453662 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  HS054_RS07335 (HS054_07285) comE 1453732..1455054 (-) 1323 WP_409042642.1 type IV pilus secretin PilQ Machinery gene
  HS054_RS07340 (HS054_07290) - 1455063..1455476 (-) 414 WP_240017829.1 hypothetical protein -
  HS054_RS07345 (HS054_07295) - 1455479..1456015 (-) 537 WP_006247935.1 hypothetical protein -
  HS054_RS07350 (HS054_07300) - 1456017..1456538 (-) 522 WP_006247934.1 hypothetical protein -
  HS054_RS07355 (HS054_07305) - 1456514..1457212 (-) 699 WP_240017830.1 competence protein ComA -
  HS054_RS07360 (HS054_07310) - 1457336..1459903 (+) 2568 WP_409042643.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 48781.42 Da        Isoelectric Point: 7.1883

>NTDB_id=468126 HS054_RS07335 WP_409042642.1 1453732..1455054(-) (comE) [Mannheimia haemolytica strain 0001]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKXEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNVN
FPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLG
LEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKNQNSTS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=468126 HS054_RS07335 WP_409042642.1 1453732..1455054(-) (comE) [Mannheimia haemolytica strain 0001]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAANAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCAG
AGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGCAAT
AGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGAGCA
AATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTTCTC
GTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTAAAT
TTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAGTGC
ATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCAAGC
AAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTAGGG
CTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTCCCA
ATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCATTG
TACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATCAAG
CGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATCTAA
TGAAAAGAGCAGCAACCGCAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-424)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.934

97.045

0.62
  comE Haemophilus influenzae Rd KW20

50.45

100

0.509
  comE Haemophilus influenzae 86-028NP

50.345

98.864

0.498
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.005

95.682

0.364
  pilQ Vibrio cholerae strain A1552

38.005

95.682

0.364
  pilQ Vibrio campbellii strain DS40M4

37.15

97.273

0.361