Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS055_RS10280 Genome accession   NZ_CP059243
Coordinates   2032321..2032803 (+) Length   160 a.a.
NCBI ID   WP_275672167.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2027321..2037803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS055_RS10255 (HS055_10210) - 2027900..2028664 (-) 765 WP_006248395.1 TSUP family transporter -
  HS055_RS10260 (HS055_10215) proB 2028664..2029752 (-) 1089 WP_409041906.1 glutamate 5-kinase -
  HS055_RS10265 (HS055_10220) folA 2029864..2030358 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS055_RS10270 (HS055_10225) - 2030477..2030707 (+) 231 WP_006248398.1 YgjV family protein -
  HS055_RS10275 (HS055_10230) radA 2030762..2032141 (-) 1380 WP_409041907.1 DNA repair protein RadA -
  HS055_RS10280 (HS055_10235) pilA 2032321..2032803 (+) 483 WP_275672167.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HS055_RS10285 (HS055_10240) - 2032824..2033267 (+) 444 Protein_1980 pilus assembly protein PilB -
  HS055_RS10290 (HS055_10245) pilC 2033273..2034469 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS055_RS10295 - 2034472..2034675 (+) 204 WP_240018335.1 hypothetical protein -
  HS055_RS10300 - 2034746..2034952 (+) 207 Protein_1983 peptidase A24 -
  HS055_RS10305 (HS055_10255) coaE 2035166..2035801 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS055_RS10310 (HS055_10260) yacG 2035783..2035968 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS055_RS10315 (HS055_10265) holA 2035969..2036994 (-) 1026 WP_409038164.1 DNA polymerase III subunit delta -
  HS055_RS10320 (HS055_10270) lptE 2037037..2037555 (-) 519 WP_409037689.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 16960.03 Da        Isoelectric Point: 8.7964

>NTDB_id=468105 HS055_RS10280 WP_275672167.1 2032321..2032803(+) (pilA) [Mannheimia haemolytica strain 0002]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQAXSASYKSDVEVCIYNSGGLDNCS
GGTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 483 bp        

>NTDB_id=468105 HS055_RS10280 WP_275672167.1 2032321..2032803(+) (pilA) [Mannheimia haemolytica strain 0002]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCTNNATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCC
GGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGG
CGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTA
GTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGC
TAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

65.625

100

0.656
  pilA Haemophilus influenzae 86-028NP

56.028

88.125

0.494
  pilA Haemophilus influenzae Rd KW20

54.545

89.375

0.487