Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS058_RS02265 Genome accession   NZ_CP059240
Coordinates   420066..420545 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415066..425545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS058_RS02225 (HS058_02185) lptE 415317..415835 (+) 519 WP_409037689.1 LPS assembly lipoprotein LptE -
  HS058_RS02230 (HS058_02190) holA 415878..416903 (+) 1026 WP_409043435.1 DNA polymerase III subunit delta -
  HS058_RS02235 (HS058_02195) yacG 416904..417089 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS058_RS02240 (HS058_02200) coaE 417071..417706 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  HS058_RS02245 - 417917..418123 (-) 207 Protein_442 peptidase A24 -
  HS058_RS02250 - 418194..418397 (-) 204 WP_240018335.1 hypothetical protein -
  HS058_RS02255 (HS058_02210) pilC 418400..419596 (-) 1197 WP_240025848.1 type II secretion system F family protein Machinery gene
  HS058_RS02260 (HS058_02215) - 419602..420045 (-) 444 Protein_445 pilus assembly protein PilB -
  HS058_RS02265 (HS058_02220) pilA 420066..420545 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS058_RS02270 (HS058_02225) radA 420725..422104 (+) 1380 WP_409041650.1 DNA repair protein RadA -
  HS058_RS02275 (HS058_02230) - 422159..422389 (-) 231 WP_006248398.1 YgjV family protein -
  HS058_RS02280 (HS058_02235) - 422508..423002 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS058_RS02285 (HS058_02240) proB 423114..424199 (+) 1086 WP_240025850.1 glutamate 5-kinase -
  HS058_RS02290 (HS058_02245) - 424199..424963 (+) 765 WP_240018340.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=468025 HS058_RS02265 WP_006248401.1 420066..420545(-) (pilA) [Mannheimia haemolytica strain 0039]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=468025 HS058_RS02265 WP_006248401.1 420066..420545(-) (pilA) [Mannheimia haemolytica strain 0039]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491