Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HS058_RS02255 Genome accession   NZ_CP059240
Coordinates   418400..419596 (-) Length   398 a.a.
NCBI ID   WP_240025848.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0039     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413400..424596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS058_RS02225 (HS058_02185) lptE 415317..415835 (+) 519 WP_409037689.1 LPS assembly lipoprotein LptE -
  HS058_RS02230 (HS058_02190) holA 415878..416903 (+) 1026 WP_409043435.1 DNA polymerase III subunit delta -
  HS058_RS02235 (HS058_02195) yacG 416904..417089 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS058_RS02240 (HS058_02200) coaE 417071..417706 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  HS058_RS02245 - 417917..418123 (-) 207 Protein_442 peptidase A24 -
  HS058_RS02250 - 418194..418397 (-) 204 WP_240018335.1 hypothetical protein -
  HS058_RS02255 (HS058_02210) pilC 418400..419596 (-) 1197 WP_240025848.1 type II secretion system F family protein Machinery gene
  HS058_RS02260 (HS058_02215) - 419602..420045 (-) 444 Protein_445 pilus assembly protein PilB -
  HS058_RS02265 (HS058_02220) pilA 420066..420545 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS058_RS02270 (HS058_02225) radA 420725..422104 (+) 1380 WP_409041650.1 DNA repair protein RadA -
  HS058_RS02275 (HS058_02230) - 422159..422389 (-) 231 WP_006248398.1 YgjV family protein -
  HS058_RS02280 (HS058_02235) - 422508..423002 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS058_RS02285 (HS058_02240) proB 423114..424199 (+) 1086 WP_240025850.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45149.17 Da        Isoelectric Point: 9.3216

>NTDB_id=468024 HS058_RS02255 WP_240025848.1 418400..419596(-) (pilC) [Mannheimia haemolytica strain 0039]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKXEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFXAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=468024 HS058_RS02255 WP_240025848.1 418400..419596(-) (pilC) [Mannheimia haemolytica strain 0039]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAANAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTNTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

53.75

100

0.54
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415