Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS061_RS05230 Genome accession   NZ_CP059237
Coordinates   953865..954344 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0583     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 948865..959344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS061_RS05190 (HS061_05085) lptE 949145..949633 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  HS061_RS05195 (HS061_05090) holA 949676..950701 (+) 1026 WP_240026304.1 DNA polymerase III subunit delta -
  HS061_RS05200 (HS061_05095) yacG 950702..950887 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS061_RS05205 (HS061_05100) coaE 950869..951504 (-) 636 WP_409041777.1 dephospho-CoA kinase -
  HS061_RS05210 - 951715..951921 (-) 207 Protein_1024 peptidase A24 -
  HS061_RS05215 - 951906..952196 (-) 291 WP_409040216.1 hypothetical protein -
  HS061_RS05220 (HS061_05110) pilC 952199..953395 (-) 1197 WP_409041649.1 type II secretion system F family protein Machinery gene
  HS061_RS05225 (HS061_05115) - 953401..953844 (-) 444 Protein_1027 pilus assembly protein PilB -
  HS061_RS05230 (HS061_05120) pilA 953865..954344 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS061_RS05235 (HS061_05125) radA 954524..955903 (+) 1380 WP_409041650.1 DNA repair protein RadA -
  HS061_RS05240 (HS061_05130) - 955958..956188 (-) 231 WP_006248398.1 YgjV family protein -
  HS061_RS05245 (HS061_05135) - 956307..956801 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS061_RS05250 (HS061_05140) proB 956913..957998 (+) 1086 WP_409041651.1 glutamate 5-kinase -
  HS061_RS05255 (HS061_05145) - 957998..958762 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=467961 HS061_RS05230 WP_006248401.1 953865..954344(-) (pilA) [Mannheimia haemolytica strain 0583]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=467961 HS061_RS05230 WP_006248401.1 953865..954344(-) (pilA) [Mannheimia haemolytica strain 0583]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491