Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HS061_RS05220 Genome accession   NZ_CP059237
Coordinates   952199..953395 (-) Length   398 a.a.
NCBI ID   WP_409041649.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0583     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 947199..958395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS061_RS05190 (HS061_05085) lptE 949145..949633 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  HS061_RS05195 (HS061_05090) holA 949676..950701 (+) 1026 WP_240026304.1 DNA polymerase III subunit delta -
  HS061_RS05200 (HS061_05095) yacG 950702..950887 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS061_RS05205 (HS061_05100) coaE 950869..951504 (-) 636 WP_409041777.1 dephospho-CoA kinase -
  HS061_RS05210 - 951715..951921 (-) 207 Protein_1024 peptidase A24 -
  HS061_RS05215 - 951906..952196 (-) 291 WP_409040216.1 hypothetical protein -
  HS061_RS05220 (HS061_05110) pilC 952199..953395 (-) 1197 WP_409041649.1 type II secretion system F family protein Machinery gene
  HS061_RS05225 (HS061_05115) - 953401..953844 (-) 444 Protein_1027 pilus assembly protein PilB -
  HS061_RS05230 (HS061_05120) pilA 953865..954344 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS061_RS05235 (HS061_05125) radA 954524..955903 (+) 1380 WP_409041650.1 DNA repair protein RadA -
  HS061_RS05240 (HS061_05130) - 955958..956188 (-) 231 WP_006248398.1 YgjV family protein -
  HS061_RS05245 (HS061_05135) - 956307..956801 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS061_RS05250 (HS061_05140) proB 956913..957998 (+) 1086 WP_409041651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45158.46 Da        Isoelectric Point: 9.4698

>NTDB_id=467960 HS061_RS05220 WP_409041649.1 952199..953395(-) (pilC) [Mannheimia haemolytica strain 0583]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLFXAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=467960 HS061_RS05220 WP_409041649.1 952199..953395(-) (pilC) [Mannheimia haemolytica strain 0583]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTTNTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

42.145

100

0.425
  pilC Haemophilus influenzae 86-028NP

41.147

100

0.415