Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS064_RS11995 Genome accession   NZ_CP059234
Coordinates   2375523..2375933 (+) Length   136 a.a.
NCBI ID   WP_409040070.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0611     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370523..2380933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS064_RS11970 (HS064_11875) - 2371034..2371798 (-) 765 WP_409039642.1 TSUP family transporter -
  HS064_RS11975 (HS064_11880) proB 2371798..2372883 (-) 1086 WP_409037686.1 glutamate 5-kinase -
  HS064_RS11980 (HS064_11885) folA 2372995..2373489 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS064_RS11985 (HS064_11890) - 2373608..2373838 (+) 231 WP_006248398.1 YgjV family protein -
  HS064_RS11990 (HS064_11895) radA 2373893..2375272 (-) 1380 WP_240019414.1 DNA repair protein RadA -
  HS064_RS11995 (HS064_11900) pilA 2375523..2375933 (+) 411 WP_409040070.1 prepilin-type cleavage/methylation domain-containing protein Machinery gene
  HS064_RS12000 (HS064_11905) - 2375954..2376397 (+) 444 Protein_2318 pilus assembly protein PilB -
  HS064_RS12005 (HS064_11910) pilC 2376403..2377599 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS064_RS12010 - 2377878..2378084 (+) 207 Protein_2320 peptidase A24 -
  HS064_RS12015 (HS064_11920) coaE 2378298..2378933 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS064_RS12020 (HS064_11925) yacG 2378915..2379100 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS064_RS12025 (HS064_11930) holA 2379101..2380126 (-) 1026 WP_409038164.1 DNA polymerase III subunit delta -
  HS064_RS12030 (HS064_11935) lptE 2380169..2380657 (-) 489 WP_409039643.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14060.49 Da        Isoelectric Point: 7.5809

>NTDB_id=467906 HS064_RS11995 WP_409040070.1 2375523..2375933(+) (pilA) [Mannheimia haemolytica strain 0611]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQAXSASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAV
TKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 411 bp        

>NTDB_id=467906 HS064_RS11995 WP_409040070.1 2375523..2375933(+) (pilA) [Mannheimia haemolytica strain 0611]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTACAAGCTNNATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATA
ACTGCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTA
ACAAAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAA
TACGATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACAC
CAAACGGCTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

63.636

88.971

0.566
  pilA Haemophilus influenzae 86-028NP

50.862

85.294

0.434
  pilA Haemophilus influenzae Rd KW20

49.573

86.029

0.426