Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I6J82_RS22705 Genome accession   NZ_CP069528
Coordinates   4760933..4761610 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain FDAARGOS_1252     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4755933..4766610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J82_RS22690 (I6J82_22690) chiA 4757291..4759993 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  I6J82_RS22695 (I6J82_22695) bfd 4760162..4760356 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I6J82_RS22700 (I6J82_22700) bfr 4760428..4760904 (+) 477 WP_000675504.1 bacterioferritin -
  I6J82_RS22705 (I6J82_22705) pilD 4760933..4761610 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I6J82_RS22710 (I6J82_22710) gspM 4761610..4762071 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  I6J82_RS22715 (I6J82_22715) gspL 4762068..4763231 (-) 1164 WP_001539911.1 type II secretion system protein GspL -
  I6J82_RS22720 (I6J82_22720) gspK 4763246..4764229 (-) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -
  I6J82_RS22725 (I6J82_22725) gspJ 4764222..4764809 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I6J82_RS22730 (I6J82_22730) gspI 4764802..4765179 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  I6J82_RS22735 (I6J82_22735) gspH 4765176..4765685 (-) 510 WP_001539909.1 type II secretion system minor pseudopilin GspH -
  I6J82_RS22740 (I6J82_22740) gspG 4765693..4766130 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=467907 I6J82_RS22705 WP_000178175.1 4760933..4761610(-) (pilD) [Escherichia coli strain FDAARGOS_1252]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=467907 I6J82_RS22705 WP_000178175.1 4760933..4761610(-) (pilD) [Escherichia coli strain FDAARGOS_1252]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387