Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HS064_RS03575 Genome accession   NZ_CP059234
Coordinates   707085..708410 (+) Length   441 a.a.
NCBI ID   WP_409039890.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0611     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 702085..713410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS064_RS03555 (HS064_03525) - 702235..704802 (-) 2568 WP_409039889.1 penicillin-binding protein 1A -
  HS064_RS03560 (HS064_03535) - 705601..706122 (+) 522 WP_006247934.1 hypothetical protein -
  HS064_RS03565 (HS064_03540) - 706124..706660 (+) 537 WP_006247935.1 hypothetical protein -
  HS064_RS03570 (HS064_03545) - 706663..707076 (+) 414 WP_006247936.1 hypothetical protein -
  HS064_RS03575 (HS064_03550) comE 707085..708410 (+) 1326 WP_409039890.1 type IV pilus secretin PilQ Machinery gene
  HS064_RS03580 (HS064_03555) nusB 708481..708894 (+) 414 WP_006247938.1 transcription antitermination factor NusB -
  HS064_RS03585 (HS064_03560) thiL 708957..709931 (+) 975 Protein_700 thiamine-phosphate kinase -
  HS064_RS03590 - 709941..710417 (+) 477 WP_409040033.1 phosphatidylglycerophosphatase A -
  HS064_RS03595 (HS064_03565) - 710424..711047 (+) 624 WP_006247941.1 LysE family transporter -
  HS064_RS03600 (HS064_03570) ruvA 711159..711764 (+) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  HS064_RS03605 (HS064_03575) ruvB 711901..712905 (+) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48915.32 Da        Isoelectric Point: 7.5467

>NTDB_id=467891 HS064_RS03575 WP_409039890.1 707085..708410(+) (comE) [Mannheimia haemolytica strain 0611]
MWRIFIYFLCGFSLAYANTISIVXKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASXEVIESLTKGSGTFLSENGYIHFDERS
NSLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNV
NFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVL
GLEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFI
KRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKNQNSTS

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=467891 HS064_RS03575 WP_409039890.1 707085..708410(+) (comE) [Mannheimia haemolytica strain 0611]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTNAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCCN
NAGAGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGC
AATAGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGA
GCAAATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTT
CTCGTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTA
AATTTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAG
TGCATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCA
AGCAAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTA
GGGCTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTC
CCAATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCA
TTGTACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATC
AAGCGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATC
TAATGAAAAGAGCAGCAACCGCAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(268-425)

Bacterial type II/III secretion system short domain (Secretin_N)

(58-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.785

97.052

0.619
  comE Haemophilus influenzae Rd KW20

50.112

100

0.506
  comE Haemophilus influenzae 86-028NP

50

98.866

0.494
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.626

95.692

0.37
  pilQ Vibrio cholerae strain A1552

38.626

95.692

0.37
  pilQ Vibrio campbellii strain DS40M4

37.442

97.506

0.365