Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS065_RS03665 Genome accession   NZ_CP059233
Coordinates   746303..746782 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0627     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741303..751782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS065_RS03640 (HS065_03625) - 741886..742650 (-) 765 WP_240028779.1 TSUP family transporter -
  HS065_RS03645 (HS065_03630) proB 742650..743735 (-) 1086 WP_240019412.1 glutamate 5-kinase -
  HS065_RS03650 (HS065_03635) folA 743847..744341 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS065_RS03655 (HS065_03640) - 744459..744689 (+) 231 WP_006248398.1 YgjV family protein -
  HS065_RS03660 (HS065_03645) radA 744744..746123 (-) 1380 WP_409037687.1 DNA repair protein RadA -
  HS065_RS03665 (HS065_03650) pilA 746303..746782 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS065_RS03670 (HS065_03655) - 746803..747246 (+) 444 Protein_723 pilus assembly protein PilB -
  HS065_RS03675 (HS065_03660) pilC 747252..748448 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS065_RS03680 - 748727..748933 (+) 207 Protein_725 peptidase A24 -
  HS065_RS03685 (HS065_03670) coaE 749147..749782 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS065_RS03690 (HS065_03675) yacG 749764..749949 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS065_RS03695 (HS065_03680) holA 749950..750975 (-) 1026 WP_409046032.1 DNA polymerase III subunit delta -
  HS065_RS03700 (HS065_03685) lptE 751018..751506 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=467869 HS065_RS03665 WP_006248401.1 746303..746782(+) (pilA) [Mannheimia haemolytica strain 0627]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=467869 HS065_RS03665 WP_006248401.1 746303..746782(+) (pilA) [Mannheimia haemolytica strain 0627]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491