Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HS065_RS03675 Genome accession   NZ_CP059233
Coordinates   747252..748448 (+) Length   398 a.a.
NCBI ID   WP_409037688.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0627     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742252..753448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS065_RS03645 (HS065_03630) proB 742650..743735 (-) 1086 WP_240019412.1 glutamate 5-kinase -
  HS065_RS03650 (HS065_03635) folA 743847..744341 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS065_RS03655 (HS065_03640) - 744459..744689 (+) 231 WP_006248398.1 YgjV family protein -
  HS065_RS03660 (HS065_03645) radA 744744..746123 (-) 1380 WP_409037687.1 DNA repair protein RadA -
  HS065_RS03665 (HS065_03650) pilA 746303..746782 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS065_RS03670 (HS065_03655) - 746803..747246 (+) 444 Protein_723 pilus assembly protein PilB -
  HS065_RS03675 (HS065_03660) pilC 747252..748448 (+) 1197 WP_409037688.1 type II secretion system F family protein Machinery gene
  HS065_RS03680 - 748727..748933 (+) 207 Protein_725 peptidase A24 -
  HS065_RS03685 (HS065_03670) coaE 749147..749782 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS065_RS03690 (HS065_03675) yacG 749764..749949 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS065_RS03695 (HS065_03680) holA 749950..750975 (-) 1026 WP_409046032.1 DNA polymerase III subunit delta -
  HS065_RS03700 (HS065_03685) lptE 751018..751506 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45158.46 Da        Isoelectric Point: 9.4698

>NTDB_id=467870 HS065_RS03675 WP_409037688.1 747252..748448(+) (pilC) [Mannheimia haemolytica strain 0627]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLXFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=467870 HS065_RS03675 WP_409037688.1 747252..748448(+) (pilC) [Mannheimia haemolytica strain 0627]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAAAAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTNTTTGCAAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

54

100

0.543
  pilC Haemophilus influenzae Rd KW20

41.895

100

0.422
  pilC Haemophilus influenzae 86-028NP

40.898

100

0.412