Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS066_RS07935 Genome accession   NZ_CP059232
Coordinates   1519083..1519490 (-) Length   135 a.a.
NCBI ID   WP_240020848.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0634     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1514083..1524490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS066_RS07900 (HS066_07820) lptE 1514329..1514847 (+) 519 WP_409037689.1 LPS assembly lipoprotein LptE -
  HS066_RS07905 (HS066_07825) holA 1514890..1515915 (+) 1026 WP_240026304.1 DNA polymerase III subunit delta -
  HS066_RS07910 (HS066_07830) yacG 1515916..1516101 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS066_RS07915 (HS066_07835) coaE 1516083..1516721 (-) 639 WP_409045446.1 dephospho-CoA kinase -
  HS066_RS07920 (HS066_07840) - 1516932..1517414 (-) 483 Protein_1541 peptidase A24 -
  HS066_RS07925 (HS066_07845) pilC 1517417..1518613 (-) 1197 WP_409041649.1 type II secretion system F family protein Machinery gene
  HS066_RS07930 (HS066_07850) - 1518619..1519062 (-) 444 Protein_1543 pilus assembly protein PilB -
  HS066_RS07935 (HS066_07855) pilA 1519083..1519490 (-) 408 WP_240020848.1 pilin Machinery gene
  HS066_RS07940 (HS066_07860) radA 1519741..1521120 (+) 1380 WP_240025849.1 DNA repair protein RadA -
  HS066_RS07945 (HS066_07865) - 1521175..1521405 (-) 231 WP_006248398.1 YgjV family protein -
  HS066_RS07950 (HS066_07870) - 1521524..1522018 (-) 495 WP_240018338.1 dihydrofolate reductase -
  HS066_RS07955 (HS066_07875) proB 1522130..1523215 (+) 1086 WP_240025850.1 glutamate 5-kinase -
  HS066_RS07960 (HS066_07880) - 1523215..1523982 (+) 768 WP_409045015.1 TSUP family transporter -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13941.60 Da        Isoelectric Point: 7.5809

>NTDB_id=467854 HS066_RS07935 WP_240020848.1 1519083..1519490(-) (pilA) [Mannheimia haemolytica strain 0634]
MIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIAVT
KGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 408 bp        

>NTDB_id=467854 HS066_RS07935 WP_240020848.1 1519083..1519490(-) (pilA) [Mannheimia haemolytica strain 0634]
ATGATCGTGATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTT
ATCGGAGTTATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACT
GCTCCGGTGGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACA
AAAGGCGTGATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATAC
GATTAGTTGGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAA
ACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(24-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

64.167

88.889

0.57
  pilA Haemophilus influenzae 86-028NP

51.304

85.185

0.437
  pilA Haemophilus influenzae Rd KW20

50

85.926

0.43