Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HS072_RS08855 Genome accession   NZ_CP059227
Coordinates   1766756..1768081 (-) Length   441 a.a.
NCBI ID   WP_409043976.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0731     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1761756..1773081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS072_RS08825 (HS072_08810) ruvB 1762265..1763269 (-) 1005 WP_006247944.1 Holliday junction branch migration DNA helicase RuvB -
  HS072_RS08830 (HS072_08815) ruvA 1763406..1764011 (-) 606 WP_006247943.1 Holliday junction branch migration protein RuvA -
  HS072_RS08835 (HS072_08820) - 1764123..1764746 (-) 624 WP_006247941.1 LysE family transporter -
  HS072_RS08840 - 1764753..1765226 (-) 474 WP_240018882.1 phosphatidylglycerophosphatase A -
  HS072_RS08845 (HS072_08825) thiL 1765236..1766210 (-) 975 Protein_1703 thiamine-phosphate kinase -
  HS072_RS08850 (HS072_08830) nusB 1766273..1766686 (-) 414 WP_006247938.1 transcription antitermination factor NusB -
  HS072_RS08855 (HS072_08835) comE 1766756..1768081 (-) 1326 WP_409043976.1 type IV pilus secretin PilQ Machinery gene
  HS072_RS08860 (HS072_08840) - 1768090..1768503 (-) 414 WP_006247936.1 hypothetical protein -
  HS072_RS08865 (HS072_08845) - 1768506..1769042 (-) 537 WP_006247935.1 hypothetical protein -
  HS072_RS08870 - 1769363..1769563 (-) 201 WP_409042321.1 hypothetical protein -
  HS072_RS08875 (HS072_08855) - 1769539..1770237 (-) 699 WP_240017830.1 competence protein ComA -
  HS072_RS08880 (HS072_08860) - 1770361..1772931 (+) 2571 WP_409043977.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48909.59 Da        Isoelectric Point: 7.5467

>NTDB_id=467811 HS072_RS08855 WP_409043976.1 1766756..1768081(-) (comE) [Mannheimia haemolytica strain 0731]
MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNMVLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQ
EDDIVYIHKKEEKAADLTPLPIMNLQNNGQNTPLVTAPKLITKTLKLHYAKASXEVIESLTKGSGTFLSENGYIHFDERS
NSLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGHYKFGGRLEGNGLNNVTNNLNV
NFPITGGASAVLQVSSINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVL
GLEVVPHLSTQNQILLDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPILGSIPFI
KRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKNQNSTS

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=467811 HS072_RS08855 WP_409043976.1 1766756..1768081(-) (comE) [Mannheimia haemolytica strain 0731]
ATGTGGCGAATTTTTATATATTTCTTATGCGGTTTTTCATTAGCCTATGCGAATACCATTTCCATAGTTTTAAAAAATGC
TCCAACTTCGCTTATTTTTACTTATTTAGCGGAAGAAACCGGTAAAAATATGGTGCTTGATGATAATATTGAAACCAAAT
CAACACTACGATTAGAAAATAAATCGGTTGACGAGATTTTTAAAACGCTTGGCAAAGTCAATAAATTATCCTTAACGCAA
GAAGATGATATTGTTTATATTCACAAAAAAGAGGAAAAAGCGGCTGACTTAACGCCTCTGCCAATAATGAATTTGCAAAA
TAATGGGCAAAATACACCGCTTGTCACCGCCCCTAAGTTAATCACAAAAACCTTGAAACTACATTATGCAAAGGCTTCCA
NNGAGGTGATTGAGTCGTTAACCAAAGGCAGCGGAACGTTTTTATCGGAAAACGGTTATATTCACTTTGATGAAAGAAGC
AATAGCCTGATTGTGAAAGACAGTGCAAAATCGCTTAAAAATATCGAGAATTTAGTAAAACAGCTCGACCAACCCACCGA
GCAAATTGCGATTGAGGCTAGAATTGTCACTATCAGTAGCGAGCATTTGCAAGAGCTTGGCGTTCGCTGGGGAATTTTTT
CTCGTGGGGCTGGGCACTATAAATTTGGCGGACGGCTGGAAGGCAATGGACTCAACAATGTCACCAATAACTTAAATGTA
AATTTTCCGATTACTGGTGGAGCATCTGCCGTATTACAGGTTTCGTCCATTAATAGCAGAGTGCTTGATTTAGAATTAAG
TGCATTAGAACAAGAAAATAGTGTGGAAATTATTGCCAGCCCACGCCTGTTAACCACCAATAAAAAGCCGGCAAGTATCA
AGCAAGGCACGGAAATTCCTTATGTGATGTACAACACCAAATCGGAAGCCACCGATGTCGAATTTAAAGAAGCGGTATTA
GGGCTTGAAGTCGTTCCACACCTTTCAACCCAAAATCAAATTCTACTCGATCTGATCGTTACCCAAAATTCGCCAAATTC
CCAATCCGGCAGTAGTGGCTTAATTACGATTGATAAACAAGAGCTAAATACGCAAGTATTTGCCAAACACGGCGAAACCA
TTGTACTTGGTGGTATTTTTCAACATTTAACCCAAAAAGGCGAAGATAAAGTGCCGATTTTAGGCTCAATTCCCTTCATC
AAGCGGTTATTTAGTCAAACTCGAGATAAAATCAGCAAAAGAGAGCTAGTCATTTTTGTGACGCCTTATATCCTTCAATC
TAATGAAAAGAGCAGCAACCGCAAAAATCAAAATAGCACATCATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(268-425)

Bacterial type II/III secretion system short domain (Secretin_N)

(58-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

63.785

97.052

0.619
  comE Haemophilus influenzae Rd KW20

50.337

100

0.508
  comE Haemophilus influenzae 86-028NP

50.229

98.866

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

38.626

95.692

0.37
  pilQ Vibrio cholerae strain A1552

38.626

95.692

0.37
  pilQ Vibrio campbellii strain DS40M4

37.442

97.506

0.365