Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HS073_RS08325 Genome accession   NZ_CP059226
Coordinates   1643234..1644430 (+) Length   398 a.a.
NCBI ID   WP_240019415.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain 0739     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1638234..1649430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS073_RS08295 (HS073_08270) proB 1638625..1639713 (-) 1089 WP_409036532.1 glutamate 5-kinase -
  HS073_RS08300 (HS073_08275) folA 1639825..1640319 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS073_RS08305 (HS073_08280) - 1640438..1640668 (+) 231 WP_006248398.1 YgjV family protein -
  HS073_RS08310 (HS073_08285) radA 1640723..1642105 (-) 1383 WP_409036533.1 DNA repair protein RadA -
  HS073_RS08315 (HS073_08290) pilA 1642285..1642764 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS073_RS08320 (HS073_08295) - 1642785..1643228 (+) 444 Protein_1598 pilus assembly protein PilB -
  HS073_RS08325 (HS073_08300) pilC 1643234..1644430 (+) 1197 WP_240019415.1 type II secretion system F family protein Machinery gene
  HS073_RS08330 - 1644707..1644913 (+) 207 Protein_1600 peptidase A24 -
  HS073_RS08335 (HS073_08310) coaE 1645127..1645762 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS073_RS08340 (HS073_08315) yacG 1645744..1645929 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS073_RS08345 (HS073_08320) holA 1645930..1646955 (-) 1026 WP_409037364.1 DNA polymerase III subunit delta -
  HS073_RS08350 (HS073_08325) lptE 1646998..1647486 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45149.17 Da        Isoelectric Point: 9.3216

>NTDB_id=467786 HS073_RS08325 WP_240019415.1 1643234..1644430(+) (pilC) [Mannheimia haemolytica strain 0739]
MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIRISRNFVLSANPXKEEVNSLLSQLALLVNSAMPLKQA
LSMLLQNCRNIRLYLWLNELIQHIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGTVLLGTLLLAVIFYLXFAKKSRL
VAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLNAHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGIN
PNVFGAEMAQMLEVGEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFDMGALVE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=467786 HS073_RS08325 WP_240019415.1 1643234..1644430(+) (pilC) [Mannheimia haemolytica strain 0739]
ATGACGAAAATCTATGAATTTCATTGGCAAGCCCAGAGCCGATTTGGGCAAAAACAGCGGGGCAAGCGGTTGGCAACGAA
TCGAGACATGTTGGAAAACCTTTTGCTGGCACAAGGTTATCAGCATATTCGGATTAGCCGAAATTTTGTGCTTTCAGCAA
ACCCGAANAAAGAAGAGGTGAATAGCTTGCTATCACAGCTTGCATTGTTGGTTAATTCTGCCATGCCGTTAAAACAGGCA
TTATCTATGTTGTTGCAAAACTGCCGAAATATCCGCCTTTATCTATGGCTTAATGAGCTGATTCAGCATATTGAAAGTGG
CTATGCTTTTTCACAATCGTTGGAGAAATTGGGTGAATTTGTGACCAATCAGGAAATTCAGCTAATCAAAATGGGCGAAC
AAAGCGGAAAACTGGGCATTATGCTGACTAATTTAGCTGAAAGCCGAGCGAAATTGGAAAAATTGGCGAAAAAAGTGAAG
AAAATCCTGTTTTACCCGGTGATTGTGTTGCTGATTTCGCTCGCTTTATCTATCGGGCTATTGGTTTTGATTGTACCGCA
GTTTGCCCAACTTTATGGCTCAAAAGACAAAACTTTACCCCTCATTACCGATATTTTATTTACCCTTTCCGAATGCTTGA
TTGAGCAAGGCACAGTATTACTTGGCACGTTACTACTAGCGGTCATTTTTTACCTGTTNTTTGCNAAAAAGAGCAGGTTA
GTCGCAAAGTGGAAAATGGCTCTATGGGCTAAAATGCCACTGTTTAGCCGAATTATTGAGCAATCTCGCATTATCTTTTT
CAGCCAAAATATTGCCTTAATGCTGAATGCTCATATTCCGCTTGATGTTGCCTTGAGGTCGTTTTTATCCGAGAAAAATG
ATGACCCGATTTTACAGCAAGAAATTGGCACTATGCTTAATTTGTTACAACAAGGCTACAAATTTTCACAAGGGATCAAC
CCGAATGTGTTTGGTGCTGAGATGGCACAAATGCTTGAGGTTGGGGAGAAAAGTGGCAATGTGGCGAAAATGTGTGAGCA
TATTAGTGAGATGTACCAACAAAAATTAGATTACCAAATTGATATTTTGTCTCAGCTATTAGAACCGATGCTAATGTTGA
TAATGGGCGTGATTGTCGGCACTATTATTGTTGGGCTGTATTTACCGATTTTTGATATGGGGGCTTTGGTGGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

53.75

100

0.54
  pilC Haemophilus influenzae Rd KW20

41.646

100

0.42
  pilC Haemophilus influenzae 86-028NP

40.648

100

0.41