Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HS073_RS08315 Genome accession   NZ_CP059226
Coordinates   1642285..1642764 (+) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica strain 0739     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1637285..1647764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HS073_RS08290 (HS073_08265) - 1637861..1638625 (-) 765 WP_006248395.1 TSUP family transporter -
  HS073_RS08295 (HS073_08270) proB 1638625..1639713 (-) 1089 WP_409036532.1 glutamate 5-kinase -
  HS073_RS08300 (HS073_08275) folA 1639825..1640319 (+) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  HS073_RS08305 (HS073_08280) - 1640438..1640668 (+) 231 WP_006248398.1 YgjV family protein -
  HS073_RS08310 (HS073_08285) radA 1640723..1642105 (-) 1383 WP_409036533.1 DNA repair protein RadA -
  HS073_RS08315 (HS073_08290) pilA 1642285..1642764 (+) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  HS073_RS08320 (HS073_08295) - 1642785..1643228 (+) 444 Protein_1598 pilus assembly protein PilB -
  HS073_RS08325 (HS073_08300) pilC 1643234..1644430 (+) 1197 WP_240019415.1 type II secretion system F family protein Machinery gene
  HS073_RS08330 - 1644707..1644913 (+) 207 Protein_1600 peptidase A24 -
  HS073_RS08335 (HS073_08310) coaE 1645127..1645762 (+) 636 WP_006248405.1 dephospho-CoA kinase -
  HS073_RS08340 (HS073_08315) yacG 1645744..1645929 (+) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  HS073_RS08345 (HS073_08320) holA 1645930..1646955 (-) 1026 WP_409037364.1 DNA polymerase III subunit delta -
  HS073_RS08350 (HS073_08325) lptE 1646998..1647486 (-) 489 WP_006248408.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=467785 HS073_RS08315 WP_006248401.1 1642285..1642764(+) (pilA) [Mannheimia haemolytica strain 0739]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=467785 HS073_RS08315 WP_006248401.1 1642285..1642764(+) (pilA) [Mannheimia haemolytica strain 0739]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491