Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I6J92_RS13755 Genome accession   NZ_CP069517
Coordinates   2888622..2890115 (-) Length   497 a.a.
NCBI ID   WP_021524963.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1262     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2883622..2895115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J92_RS13725 (I6J92_13725) gspK 2884416..2885393 (-) 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -
  I6J92_RS13730 (I6J92_13730) gspJ 2885390..2885995 (-) 606 WP_001542359.1 type II secretion system minor pseudopilin GspJ -
  I6J92_RS13735 (I6J92_13735) gspI 2885992..2886363 (-) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  I6J92_RS13740 (I6J92_13740) gspH 2886360..2886923 (-) 564 WP_001119494.1 type II secretion system minor pseudopilin GspH -
  I6J92_RS13745 (I6J92_13745) gspG 2886927..2887382 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  I6J92_RS13750 (I6J92_13750) gspF 2887399..2888622 (-) 1224 WP_001173441.1 type II secretion system inner membrane protein GspF -
  I6J92_RS13755 (I6J92_13755) pilB/pilB1 2888622..2890115 (-) 1494 WP_021524963.1 type II secretion system ATPase GspE Machinery gene
  I6J92_RS13760 (I6J92_13760) gspD 2890115..2892175 (-) 2061 WP_000498830.1 type II secretion system secretin GspD -
  I6J92_RS13765 (I6J92_13765) gspC 2892205..2893164 (-) 960 WP_024188174.1 type II secretion system protein GspC -
  I6J92_RS13770 (I6J92_13770) gspS2 2893182..2893592 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  I6J92_RS13775 (I6J92_13775) pilD 2893658..2894467 (-) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55191.31 Da        Isoelectric Point: 5.9081

>NTDB_id=467693 I6J92_RS13755 WP_021524963.1 2888622..2890115(-) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1262]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQQSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=467693 I6J92_RS13755 WP_021524963.1 2888622..2890115(-) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1262]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGCCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGTTGATGGAAGATCTCGGTTCCGCCGAGGACTTCTTTACCCTCGCAGAAGAGCTGCCAGAAACGGAAGA
TTTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTAGACGGAACGCTACATGAAATGCTGCGT
CCCGGGCGCAAACTAGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGTCATGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGCGTCGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCCCAGACTGCCGCCAGCCTGCACCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCGCGTACTGTCACGCTTTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGGCGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACAGTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

44.111

87.123

0.384
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362