Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I6J88_RS09935 Genome accession   NZ_CP069500
Coordinates   1986492..1987985 (-) Length   497 a.a.
NCBI ID   WP_016238555.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1258     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1981492..1992985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J88_RS09905 (I6J88_09910) gspK 1982286..1983263 (-) 978 WP_016238551.1 type II secretion system minor pseudopilin GspK -
  I6J88_RS09910 (I6J88_09915) gspJ 1983260..1983865 (-) 606 WP_016238552.1 type II secretion system minor pseudopilin GspJ -
  I6J88_RS09915 (I6J88_09920) gspI 1983862..1984233 (-) 372 WP_000820112.1 type II secretion system minor pseudopilin GspI -
  I6J88_RS09920 (I6J88_09925) gspH 1984230..1984793 (-) 564 WP_016238553.1 type II secretion system minor pseudopilin GspH -
  I6J88_RS09925 (I6J88_09930) gspG 1984797..1985252 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  I6J88_RS09930 (I6J88_09935) gspF 1985269..1986492 (-) 1224 WP_016238554.1 type II secretion system inner membrane protein GspF -
  I6J88_RS09935 (I6J88_09940) pilB/pilB1 1986492..1987985 (-) 1494 WP_016238555.1 type II secretion system ATPase GspE Machinery gene
  I6J88_RS09940 (I6J88_09945) gspD 1987985..1990045 (-) 2061 WP_000498829.1 type II secretion system secretin GspD -
  I6J88_RS09945 (I6J88_09950) gspC 1990075..1991034 (-) 960 WP_024187628.1 type II secretion system protein GspC -
  I6J88_RS09950 (I6J88_09955) gspS2 1991052..1991462 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  I6J88_RS09955 (I6J88_09960) pilD 1991528..1992337 (-) 810 WP_016238557.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55230.35 Da        Isoelectric Point: 6.0084

>NTDB_id=467511 I6J88_RS09935 WP_016238555.1 1986492..1987985(-) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1258]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDDAEFEQRLNAVWQRDSS
ETRQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELLVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=467511 I6J88_RS09935 WP_016238555.1 1986492..1987985(-) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1258]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGACGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGACTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGCAAGCTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
ACAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACGTGCGCGTCTCCACCATGCCTTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACAGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACTGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACTGCCATCCATGAGCTGCTCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45.25

80.483

0.364