Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I6J79_RS20825 Genome accession   NZ_CP069459
Coordinates   4293859..4295352 (+) Length   497 a.a.
NCBI ID   WP_000249324.1    Uniprot ID   -
Organism   Escherichia coli strain FDAARGOS_1249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4288859..4300352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J79_RS20805 (I6J79_20805) pilD 4289507..4290316 (+) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene
  I6J79_RS20810 (I6J79_20810) gspS2 4290382..4290792 (+) 411 WP_016231198.1 type II secretion system pilot lipoprotein GspS-beta -
  I6J79_RS20815 (I6J79_20815) gspC 4290810..4291769 (+) 960 WP_024195261.1 type II secretion system protein GspC -
  I6J79_RS20820 (I6J79_20820) gspD 4291799..4293859 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  I6J79_RS20825 (I6J79_20825) pilB/pilB1 4293859..4295352 (+) 1494 WP_000249324.1 type II secretion system ATPase GspE Machinery gene
  I6J79_RS20830 (I6J79_20830) gspF 4295352..4296575 (+) 1224 WP_001173440.1 type II secretion system inner membrane protein GspF -
  I6J79_RS20835 (I6J79_20835) gspG 4296592..4297047 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  I6J79_RS20840 (I6J79_20840) gspH 4297051..4297614 (+) 564 WP_016231196.1 type II secretion system minor pseudopilin GspH -
  I6J79_RS20845 (I6J79_20845) gspI 4297611..4297982 (+) 372 WP_000820105.1 type II secretion system minor pseudopilin GspI -
  I6J79_RS20850 (I6J79_20850) gspJ 4297979..4298584 (+) 606 WP_001301078.1 type II secretion system minor pseudopilin GspJ -
  I6J79_RS20855 (I6J79_20855) gspK 4298581..4299558 (+) 978 WP_000631652.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55252.46 Da        Isoelectric Point: 6.0390

>NTDB_id=467206 I6J79_RS20825 WP_000249324.1 4293859..4295352(+) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1249]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDMRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQALPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=467206 I6J79_RS20825 WP_000249324.1 4293859..4295352(+) (pilB/pilB1) [Escherichia coli strain FDAARGOS_1249]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGATTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATCGACATGCGTGTCTCCACCATGCCCTCCGCTTGGGGTG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGTATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGGGTAGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGGCCCTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAATGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio cholerae strain A1552

46.717

79.678

0.372
  pilF Thermus thermophilus HB27

45

80.483

0.362