Detailed information NTG0467002

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H0S59_RS04705
• Genome accession: NZ_CP059078
• Coordinates: 965007..966125 (-)
• Length: 372 a.a.
• NCBI ID: WP_002052019.1
• Uniprot ID: A0A1V0YUM2
• Organism: Acinetobacter nosocomialis strain WM98B
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 960007..971125

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0S59_RS04675 (H0S59_04675)	-	960835..961683 (+)	849	WP_022575107.1	class II glutamine amidotransferase	-
H0S59_RS04680 (H0S59_04680)	-	961936..962391 (+)	456	WP_000782976.1	bacteriohemerythrin	-
H0S59_RS04685 (H0S59_04685)	-	962544..963611 (+)	1068	WP_033846680.1	membrane protein	-
H0S59_RS04690 (H0S59_04690)	-	963611..963934 (+)	324	WP_002052062.1	RnfH family protein	-
H0S59_RS04695 (H0S59_04695)	-	963990..964388 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H0S59_RS04700 (H0S59_04700)	fur	964500..964937 (+)	438	WP_002052029.1	ferric iron uptake transcriptional regulator	-
H0S59_RS04705 (H0S59_04705)	pilU	965007..966125 (-)	1119	WP_002052019.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H0S59_RS04710 (H0S59_04710)	pilT	966153..967190 (-)	1038	WP_006581387.1	type IV pilus twitching motility protein PilT	Machinery gene
H0S59_RS04715 (H0S59_04715)	-	967317..968009 (+)	693	WP_032010894.1	YggS family pyridoxal phosphate-dependent enzyme	-
H0S59_RS04720 (H0S59_04720)	-	968117..968959 (+)	843	WP_033846643.1	undecaprenyl-diphosphate phosphatase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41980.18 Da
• Isoelectric Point: 6.6944

>NTDB_id=467002 H0S59_RS04705 WP_002052019.1 965007..966125(-) (pilU) [Acinetobacter nosocomialis strain WM98B]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPTVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLANEGPDQITDEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=467002 H0S59_RS04705 WP_002052019.1 965007..966125(-) (pilU) [Acinetobacter nosocomialis strain WM98B]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGCCTTTCAGGTGAAGTGATTGGACAGCTCTTACATTCCA
TCATGAGTGAAAAACAACGTAAAGAATTTGCAGAGACTCGTGAATGTAACTTTGCCATCATGAACCGCGATAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGTGACATGCCGGGTATGGTACTACGTCGAATTGAAACGAAAATTCCTTCAAT
TGATGACTTACAGTTGCCGACTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGTGCAACAG
GTACAGGTAAATCGACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAG
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGCATTATTACTCAACGTGAAGTCGGTATTGATACGGACTCATTCGA
AATTGCGTTAAAAAACACGTTACGACAAGCGCCAGATGTGATCTTAATTGGTGAAATCCGCTCTCGTGAAGTCATGGACT
ACGCAATTGGCTTTGCTGAAACAGGACACCTTGTATTAGCTACAATGCATGCCAACAATGCCAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCTCTTAACTTAAAAGCCATGATTGCACA
ACAGCTTATTCCAACACCAGATGGTAATTCACGCCGTGCAGCGATTGAGATCTTAATTAACTCACCATTAATTTCAGACT
ATATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTGATGAAACGCTCACGTGAGCTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATACAAAGCAGGCCAAATTACTTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTAACGATTAAACTTGCAAATGAAGGGCCTGATCAAATTACAGACGAAAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A1V0YUM2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.254	95.43	0.403