Detailed information NTG0466638

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H0N28_RS04280
• Genome accession: NZ_CP059041
• Coordinates: 914988..916106 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain ATCC 17978
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 909988..921106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H0N28_RS04250 (H0N28_04250)	-	910817..911665 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
H0N28_RS04255 (H0N28_04255)	-	911918..912373 (+)	456	WP_000782976.1	bacteriohemerythrin	-
H0N28_RS04260 (H0N28_04260)	-	912526..913593 (+)	1068	WP_004748097.1	hypothetical protein	-
H0N28_RS04265 (H0N28_04265)	-	913593..913916 (+)	324	WP_000442590.1	RnfH family protein	-
H0N28_RS04270 (H0N28_04270)	-	913971..914369 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
H0N28_RS04275 (H0N28_04275)	fur	914481..914918 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
H0N28_RS04280 (H0N28_04280)	pilU	914988..916106 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H0N28_RS04285 (H0N28_04285)	pilT	916134..917171 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
H0N28_RS04290 (H0N28_04290)	-	917298..917990 (+)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=466638 H0N28_RS04280 WP_000347034.1 914988..916106(-) (pilU) [Acinetobacter baumannii strain ATCC 17978]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=466638 H0N28_RS04280 WP_000347034.1 914988..916106(-) (pilU) [Acinetobacter baumannii strain ATCC 17978]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39