Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H0H15_RS05840 Genome accession   NZ_CP058995
Coordinates   1268970..1269404 (-) Length   144 a.a.
NCBI ID   WP_014668227.1    Uniprot ID   -
Organism   Pasteurella multocida strain 671/90     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1263970..1274404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0H15_RS05810 (H0H15_05810) - 1264534..1264803 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  H0H15_RS05815 (H0H15_05815) yacG 1264803..1265006 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  H0H15_RS05820 (H0H15_05820) coaE 1265017..1265637 (-) 621 WP_005751078.1 dephospho-CoA kinase -
  H0H15_RS05825 (H0H15_05825) - 1265652..1266374 (-) 723 WP_016534448.1 A24 family peptidase -
  H0H15_RS05830 (H0H15_05830) - 1266374..1267585 (-) 1212 WP_014668225.1 type II secretion system F family protein -
  H0H15_RS05835 (H0H15_05835) pilB 1267585..1268970 (-) 1386 WP_014668226.1 GspE/PulE family protein Machinery gene
  H0H15_RS05840 (H0H15_05840) pilA 1268970..1269404 (-) 435 WP_014668227.1 prepilin peptidase-dependent pilin Machinery gene
  H0H15_RS05845 (H0H15_05845) ampD 1269565..1270131 (+) 567 WP_014668228.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H0H15_RS05850 (H0H15_05850) rppH 1270717..1271184 (+) 468 Protein_1125 RNA pyrophosphohydrolase -
  H0H15_RS05855 (H0H15_05855) - 1271316..1272110 (+) 795 WP_005719128.1 sulfite exporter TauE/SafE family protein -
  H0H15_RS05860 (H0H15_05860) lgt 1272120..1272923 (+) 804 WP_014668230.1 prolipoprotein diacylglyceryl transferase -
  H0H15_RS05865 (H0H15_05865) - 1272929..1273780 (+) 852 WP_014668231.1 thymidylate synthase -
  H0H15_RS05870 (H0H15_05870) tadA 1273807..1274295 (+) 489 WP_016534447.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15084.52 Da        Isoelectric Point: 9.1281

>NTDB_id=466464 H0H15_RS05840 WP_014668227.1 1268970..1269404(-) (pilA) [Pasteurella multocida strain 671/90]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTPSGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=466464 H0H15_RS05840 WP_014668227.1 1268970..1269404(-) (pilA) [Pasteurella multocida strain 671/90]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACACCGAGTGGTAACGCTGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA/pilA1 Eikenella corrodens VA1

38.129

96.528

0.368