Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HZT25_RS20695 Genome accession   NZ_CP058886
Coordinates   4254223..4254759 (-) Length   178 a.a.
NCBI ID   WP_000168305.1    Uniprot ID   A0A9P2PPK2
Organism   Shigella flexneri strain STLEFF_33     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4241942..4279212 4254223..4254759 within 0


Gene organization within MGE regions


Location: 4241942..4279212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZT25_RS20660 (HZT25_20555) - 4241942..4242223 (+) 282 WP_001295689.1 YjcB family protein -
  HZT25_RS20665 (HZT25_20560) - 4242631..4243452 (+) 822 WP_001272932.1 abortive infection system antitoxin AbiGi family protein -
  HZT25_RS20670 (HZT25_20565) avs4 4243991..4248754 (-) 4764 WP_000240574.1 AVAST type 4 anti-phage nuclease Avs4 -
  HZT25_RS20675 (HZT25_20570) - 4248830..4249249 (-) 420 WP_001062122.1 hypothetical protein -
  HZT25_RS20680 (HZT25_20575) - 4249242..4251275 (-) 2034 WP_000807722.1 hypothetical protein -
  HZT25_RS20685 (HZT25_20580) - 4251292..4252824 (-) 1533 WP_001061276.1 site-specific integrase -
  HZT25_RS20690 (HZT25_20585) - 4252817..4254064 (-) 1248 WP_000414651.1 site-specific integrase -
  HZT25_RS20695 (HZT25_20590) ssb 4254223..4254759 (-) 537 WP_000168305.1 single-stranded DNA-binding protein SSB1 Machinery gene
  HZT25_RS20700 (HZT25_20595) uvrA 4255013..4257835 (+) 2823 WP_000357740.1 excinuclease ABC subunit UvrA -
  HZT25_RS20705 (HZT25_20600) - 4257870..4258226 (-) 357 WP_000155657.1 MmcQ/YjbR family DNA-binding protein -
  HZT25_RS20710 (HZT25_20605) - 4258230..4258646 (-) 417 WP_000270375.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  HZT25_RS20715 (HZT25_20610) aphA 4258757..4259470 (-) 714 WP_001226928.1 acid phosphatase AphA -
  HZT25_RS20720 (HZT25_20615) - 4259872..4260075 (+) 204 WP_001321562.1 protein YjbS -
  HZT25_RS20725 (HZT25_20620) tyrB 4260577..4261770 (-) 1194 WP_000486985.1 aromatic amino acid transaminase -
  HZT25_RS20730 (HZT25_20625) alr 4262023..4263102 (-) 1080 WP_001147328.1 alanine racemase -
  HZT25_RS20735 (HZT25_20630) dnaB 4263155..4264570 (-) 1416 WP_000918363.1 replicative DNA helicase -
  HZT25_RS20740 (HZT25_20635) - 4264653..4265636 (+) 984 WP_000235508.1 quinone oxidoreductase -
  HZT25_RS20745 (HZT25_20640) pspG 4265802..4266044 (-) 243 WP_000891404.1 envelope stress response protein PspG -
  HZT25_RS20750 (HZT25_20645) dusA 4266178..4267215 (-) 1038 WP_001298868.1 tRNA dihydrouridine(20/20a) synthase DusA -
  HZT25_RS20755 (HZT25_20650) - 4267578..4268285 (-) 708 WP_001311303.1 DUF2713 domain-containing protein -
  HZT25_RS23175 yjbL 4268309..4268563 (-) 255 WP_000912571.1 protein YjbL -
  HZT25_RS20760 (HZT25_20655) zur 4268881..4269396 (+) 516 WP_001295691.1 zinc uptake transcriptional repressor Zur -
  HZT25_RS20765 (HZT25_20660) - 4269438..4269647 (-) 210 WP_001030593.1 CsbD family protein -
  HZT25_RS20770 (HZT25_20665) dinF 4269763..4271088 (-) 1326 WP_001326646.1 MATE family efflux transporter DinF -
  HZT25_RS20775 (HZT25_20670) lexA 4271161..4271769 (-) 609 WP_000646078.1 transcriptional repressor LexA -
  HZT25_RS20780 (HZT25_20675) dgkA 4271879..4272247 (-) 369 WP_000002907.1 diacylglycerol kinase -
  HZT25_RS20785 (HZT25_20680) plsB 4272418..4274841 (+) 2424 WP_000017354.1 glycerol-3-phosphate 1-O-acyltransferase PlsB -
  HZT25_RS20790 (HZT25_20685) ubiA 4274996..4275868 (-) 873 WP_000455227.1 4-hydroxybenzoate octaprenyltransferase -
  HZT25_RS20795 (HZT25_20690) ubiC 4275881..4276378 (-) 498 WP_001326644.1 chorismate lyase -
  HZT25_RS20800 (HZT25_20695) - 4276601..4278055 (-) 1455 Protein_4092 SopA family protein -
  HZT25_RS20805 (HZT25_20700) - 4278112..4278809 (+) 698 WP_095033700.1 IS1-like element IS1B family transposase -

Sequence


Protein


Download         Length: 178 a.a.        Molecular weight: 18975.00 Da        Isoelectric Point: 5.2358

>NTDB_id=465842 HZT25_RS20695 WP_000168305.1 4254223..4254759(-) (ssb) [Shigella flexneri strain STLEFF_33]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI
EGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
PAAPSNEPPMDFDDDIPF

Nucleotide


Download         Length: 537 bp        

>NTDB_id=465842 HZT25_RS20695 WP_000168305.1 4254223..4254759(-) (ssb) [Shigella flexneri strain STLEFF_33]
ATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGG
TGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTG
AATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATC
GAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACACCACAGAAGTCGTGGTGAACGTTGG
CGGCACCATGCAGATGCTGGGTGGTCGTCAGGGTGGTGGCGCTCCGGCAGGTGGCAATATCGGTGGTGGTCAGCCGCAGG
GCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCT
CCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

74.444

100

0.753

  ssb Glaesserella parasuis strain SC1401

57.923

100

0.596

  ssb Neisseria meningitidis MC58

48.066

100

0.489

  ssb Neisseria gonorrhoeae MS11

48.066

100

0.489